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Literature summary for 3.4.23.B5 extracted from
- Characterization of the murine leukemia virus protease and its comparison with the human immunodeficiency virus type 1 protease (2006), J. Gen. Virol., 87, 1321-1330.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the enzyme with C-terminal GGSIEGR | Moloney murine leukemia virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of truncation mutant protein Gag_DELTA1 | Moloney murine leukemia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amprenavir | - |
Moloney murine leukemia virus |  |
| DMP323 | - |
Moloney murine leukemia virus | |
| indinavir | - |
Moloney murine leukemia virus | |
| KH-164 | - |
Moloney murine leukemia virus | |
| additional information | inhibition profile | Moloney murine leukemia virus | |
| nelfinavir | - |
Moloney murine leukemia virus | |
| ritonavir | - |
Moloney murine leukemia virus | |
| saquinavir | - |
Moloney murine leukemia virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
TSTLLIENSS | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.04 | - |
VQALVLTQ | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.05 | - |
MSKLLATVVS | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.05 | - |
TQTSLLIENSS | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.22 | - |
TSQAFPLRAG | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.26 | - |
PRSSLYPALTP | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gag polyprotein + H2O | Moloney murine leukemia virus | processing to mature proteins, autolysis of the retropepsin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moloney murine leukemia virus | - |
MLV | - |
| Moloney murine leukemia virus MLV | - |
MLV | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by acetobne precipitation and extraction, overview, recombinnat enzyme with C-terminal GGSIEGR | Moloney murine leukemia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Arg-Glu(EDANS)-Ser-Gln-Ala-Phe-Pro-Leu-Arg-Ala-Lys(dansyl)-Arg-OH + H2O | fluorescent substrate, contains a p12/CA cleavage site | Moloney murine leukemia virus | Arg-Glu(EDANS)-Ser-Gln-Ala-Phe + Pro-Leu-Arg-Ala-Lys(dansyl)-Arg-OH | - |
? | |
| Gag polyprotein + H2O | processing to mature proteins, autolysis of the retropepsin | Moloney murine leukemia virus | ? | - |
? | |
| Gag polyprotein + H2O | autolysis of the retropepsin from the precursor protein | Moloney murine leukemia virus | ? | - |
? | |
| Lys-Ala-Arg-Val-Nle-Phe(4-nitro)-Glu-Ala-Nle-amide + H2O | commercial chromogenic substrate | Moloney murine leukemia virus | ? | - |
? | |
| additional information | activity of recombinant enzyme with recombinant Gag protein fragments, overview | Moloney murine leukemia virus | ? | - |
? | |
| additional information | activity of recombinant enzyme with recombinant Gag protein fragments, overview | Moloney murine leukemia virus MLV | ? | - |
? | |
| MSKLLATVVS + H2O | contains a CA/NC cleavage site | Moloney murine leukemia virus | MSKLL + ATVVS | - |
? | |
| MSKLLATVVS + H2O | contains a CA/NC cleavage site | Moloney murine leukemia virus MLV | MSKLL + ATVVS | - |
? | |
| PLQVLTLNIERR + H2O | contains a PR/RT cleavage site | Moloney murine leukemia virus | PLQVL + TLNIERR | - |
? | |
| PRSSLYPALTP + H2O | contains a MA/p12 cleavage site, low activity | Moloney murine leukemia virus | PRSSLY + PALTP | - |
? | |
| TQTSLLIENSS + H2O | contains a NC/PR cleavage site | Moloney murine leukemia virus | TQTSLL + IENSS | - |
? | |
| TSQAFPLRAG + H2O | contains a p12/CA cleavage site, low activity | Moloney murine leukemia virus | TSQAF + PLRAG | - |
? | |
| TSQAFPLRAG + H2O | contains a p12/CA cleavage site, low activity | Moloney murine leukemia virus MLV | TSQAF + PLRAG | - |
? | |
| TSTLLIENSS + H2O | contains a RT/IN cleavage site | Moloney murine leukemia virus | TSTLL + IENSS | - |
? | |
| TSTLLIENSS + H2O | contains a RT/IN cleavage site | Moloney murine leukemia virus MLV | TSTLL + IENSS | - |
? | |
| VQALVLTQ + H2O | contains a p12E/p2E cleavage site | Moloney murine leukemia virus | VQAL + VLTQ | - |
? | |
| VQALVLTQ + H2O | contains a p12E/p2E cleavage site | Moloney murine leukemia virus MLV | VQAL + VLTQ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MLV PR | - |
Moloney murine leukemia virus |
| murine leukemia virus protease | - |
Moloney murine leukemia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Moloney murine leukemia virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
Arg-Glu(EDANS)-Ser-Gln-Ala-Phe-Pro-Leu-Arg-Ala-Lys(dansyl)-Arg-OH | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.12 | - |
TQTSLLIENSS | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.17 | - |
TSTLLIENSS | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.29 | - |
MSKLLATVVS | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.35 | - |
VQALVLTQ | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.38 | - |
TSQAFPLRAG | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.53 | - |
PRSSLYPALTP | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Moloney murine leukemia virus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000014 | - |
DMP323 | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.00000021 | - |
indinavir | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.00000041 | - |
ritonavir | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.0000019 | - |
KH-164 | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.000002 | - |
nelfinavir | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
| 0.000028 | - |
saquinavir | pH 5.6, 37°C, recombinant enzyme | Moloney murine leukemia virus | |
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Release 2023.1 (January 2023)
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