Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli fused to TrpE or to glutathione S-transferase from Schistosoma japonicum | Moloney murine leukemia virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis | Moloney murine leukemia virus | |
0.261 | - |
VSQNYPIVQ | - |
Moloney murine leukemia virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activates, optimal at 3 M | Moloney murine leukemia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13315 | - |
2 * 13315, sequence calculation, homodimer, structure molecular modeling | Moloney murine leukemia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gag polyprotein + H2O | Moloney murine leukemia virus | processing to mature proteins, autolysis of the retropepsin | ? | - |
? | |
Gag-Pol polyprotein + H2O | Moloney murine leukemia virus | processing to mature proteins, autolysis of the retropepsin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moloney murine leukemia virus | - |
Moloney MLV | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme performs autolytic processing of precursor protein | Moloney murine leukemia virus |
Purification (Comment) | Organism |
---|---|
native enzyme partially, recombinant enzyme to homogeneity from Escherichia coli | Moloney murine leukemia virus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
processing of viral polyprotein. The retroviral protease is essential for virus replication, by processing of viral Gag and Gag-Pol polyproteins | substrate binding pocket residues are His37, Val39, and Ala57 | Moloney murine leukemia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gag polyprotein + H2O | - |
Moloney murine leukemia virus | ? | - |
? | |
Gag polyprotein + H2O | processing to mature proteins, autolysis of the retropepsin | Moloney murine leukemia virus | ? | - |
? | |
Gag-Pol polyprotein + H2O | - |
Moloney murine leukemia virus | ? | - |
? | |
Gag-Pol polyprotein + H2O | processing to mature proteins, autolysis of the retropepsin | Moloney murine leukemia virus | ? | - |
? | |
additional information | cleavage site and substrate specificity, overview | Moloney murine leukemia virus | ? | - |
? | |
VSQNYPIVQ + H2O | a HIV-1 retropepsin substrate | Moloney murine leukemia virus | VSQNY + PIVQ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 13315, sequence calculation, homodimer, structure molecular modeling | Moloney murine leukemia virus |
Synonyms | Comment | Organism |
---|---|---|
MLV protease | - |
Moloney murine leukemia virus |
MLV retropepsin | - |
Moloney murine leukemia virus |
Moloney MLV retropepsin | - |
Moloney murine leukemia virus |
Moloney murine leukemia virus retropepsin | - |
Moloney murine leukemia virus |
More | the enzyme belongs to the A2 peptidase family | Moloney murine leukemia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Moloney murine leukemia virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
VSQNYPIVQ | pH 6.0, 37°C | Moloney murine leukemia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Moloney murine leukemia virus |