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Literature summary for 3.4.23.B4 extracted from

  • Fumian, T.; Tuipulotu, D.; Netzler, N.; Lun, J.; Russo, A.; Yan, G.; White, P.
    Potential therapeutic agents for feline calicivirus infection (2018), Viruses, 10, 433 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B4

Cloned(Commentary)

Cloned (Comment) Organism
ORF1, recombinant expression of the C-terminally His-tagged viral polyprotein in Escherichia coli strain BL21 (DE3), quantitative RT-PCR expression analysis feline calicivirus

Inhibitors

Inhibitors Comment Organism Structure
chymostatin slight inhibition feline calicivirus
GC376 75% inhibition at 0.05 mM feline calicivirus
additional information using cell culture assays, PPNDS, quercetagetin and GC376 do not display antiviral effects, while nitazoxanide and 2'-C-methylcytidine (2CMC) are potent inhibitors of FCV replication feline calicivirus
nitazoxanide slight inhibition feline calicivirus
PPNDS
-
 feline calicivirus
quercetagetin
-
 feline calicivirus
rupintrivir slight inhibition feline calicivirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics feline calicivirus
0.0335
-
 Dabcyl-FRLEADDG-Edans pH 7.5, 37°C, recombinant enzyme feline calicivirus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl the protease activity is not inhibited with increasing NaCl concentrations up to 130 mM feline calicivirus

Organism

Organism UniProt Comment Textmining
feline calicivirus Q66914 genome polyprotein; FCV
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged viral polyprotein from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration feline calicivirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dabcyl-FRLEADDG-Edans + H2O
-
 feline calicivirus Dabcyl-FRLE + ADDG-Edans
-
 ?

Subunits

Subunits Comment Organism
? x * 78000, recombinant C-terminally His-tagged viral polyprotein, SDS-PAGE feline calicivirus

Synonyms

Synonyms Comment Organism
FCV protease
-
 feline calicivirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at feline calicivirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at feline calicivirus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0027
-
 pH 7.5, 37°C, recombinant enzyme feline calicivirus PPNDS
0.0028
-
 pH 7.5, 37°C, recombinant enzyme feline calicivirus quercetagetin
0.0187
-
 pH 7.5, 37°C, recombinant enzyme feline calicivirus GC376