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Literature summary for 3.4.23.B3 extracted from

  • Powell, D.J.; Bur, D.; Wlodawer, A.; Gustchina, A.; Payne, S.L.; Dunn, B.M.; Kay, J.
    Expression, characterisation and mutagenesis of the aspartic proteinase from equine infectious anaemia virus (1996), Eur. J. Biochem., 241, 664-674.
    View publication on PubMed
Search for more literature for 3.4.23.B3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli equine infectious anemia virus

Protein Variants

Protein Variants Comment Organism
I54G mutant enzyme is only marginally different from wild-type enzyme in its susceptibility to most of the inhibitors of the HIV-1/HIV-2 proteinases. LP-149 shows a ninefold reduction in potency against the mutant enzyme. Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn is cleaved less efficiently than by the wild-type enzyme. The ability to hydrolyze Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu is lowered by at least 100fold compared to wild-type enzyme equine infectious anemia virus
T30D mutant enzyme is only marginally different from wild-type enzyme in its susceptibility to most of the inhibitors of the HIV-1/HIV-2 proteinases, except that HBY-793 has a potency that is reduced by ten-fold equine infectious anemia virus

Inhibitors

Inhibitors Comment Organism Structure
ABT-538
-
 equine infectious anemia virus
DMP-323
-
 equine infectious anemia virus
HBY-793
-
 equine infectious anemia virus
LP-149
-
 equine infectious anemia virus
Ro32-2175
-
 equine infectious anemia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.01
-
 Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.01
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.01
-
 Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu pH 4.7, wild-type enzyme equine infectious anemia virus
0.01
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.02
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme and mutant enzyme I54G equine infectious anemia virus
0.02
-
 Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
0.025
-
 Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.03
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
0.035
-
 Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.035
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.04
-
 Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.04
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.04
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
0.045
-
 Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.05
-
 Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.05
-
 Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
0.06
-
 Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.06
-
 Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
0.07
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
0.07
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
0.075
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.075
-
 Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.1
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.1
-
 Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn pH 4.7, wild-type enzyme and mutant enzyme T30D equine infectious anemia virus
0.105
-
 Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.165
-
 Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.205
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.21
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.55
-
 Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 x * 12000, SDS-PAGE equine infectious anemia virus

Organism

Organism UniProt Comment Textmining
equine infectious anemia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 equine infectious anemia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu + H2O
-
 equine infectious anemia virus Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu + p-nitrophenylalanine-Ala-Lys-Ala-Leu
-
 ?
Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Ile-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
-
 ?
Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Leu-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2
-
 ?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2
-
 ?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2
-
 ?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
-
 ?
Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-2-aminohexanoic acid + p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2
-
 ?
Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-Asn + p-nitrophenylalanine-Glu-Ala-Gly-NH2
-
 ?
Lys-Ala-Arg-Val-L-2-naphthylalanine-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-L-2-naphthylalanine + p-nitrophenylalanine-Glu-Ala-Gly-NH2
-
 ?
Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-Leu + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
-
 ?
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-Phe + p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2
-
 ?
Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-Phe + p-nitrophenylalanine-Glu-Ala-Gly-NH2
-
 ?
Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-Trp + p-nitrophenylalanine-Glu-Ala-Gly-NH2
-
 ?
Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 + H2O
-
 equine infectious anemia virus Lys-Ala-Arg-Val-Tyr + p-nitrophenylalanine-Glu-Ala-Gly-NH2
-
 ?
Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn + H2O
-
 equine infectious anemia virus Pro-Ser-Glu-Glu-Tyr + Pro-Ile-2-aminohexanoic acid-Ile-Asn
-
 ?

Subunits

Subunits Comment Organism
? x * 12000, SDS-PAGE equine infectious anemia virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Lys-Ala-Arg-Val-Asn-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.2
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.3
-
 Lys-Ala-Arg-Val-Tyr-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.5
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.5
-
 Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.6
-
 Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.8
-
 Lys-Ala-Arg-Val-Trp-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
0.8
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
0.8
-
 Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
1
-
 Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
1
-
 Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
1
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
1.3
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
1.3
-
 Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
1.3
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
1.4
-
 Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
1.5
-
 Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, mutant enzyme I54G equine infectious anemia virus
1.5
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Asn-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
1.6
-
 Lys-Ala-Arg-Val-Nal-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
1.7
-
 Pro-Ser-Glu-Glu-Tyr-Pro-Ile-2-aminohexanoic acid-Ile-Asn pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
1.9
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
1.9
-
 Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
2.2
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
2.5
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
2.6
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Gln-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
3
-
 Gln-Lys-2-aminohexanoic acid-2-aminohexanoic acid-Leu-p-nitrophenylalanine-Ala-Lys-Ala-Leu pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
3.1
-
 Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
4.2
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
4.2
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Thr-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
6.3
-
 Lys-Ala-Arg-Leu-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
7.2
-
 Lys-Ala-Arg-Ile-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-NH2 pH 4.7, 25°C, mutant enzyme T30D equine infectious anemia virus
8.2
-
 Lys-Ala-Arg-Val-2-aminohexanoic acid-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
12.5
-
 Lys-Ala-Arg-Val-Leu-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus
12.5
-
 Lys-Ala-Arg-Val-Phe-p-nitrophenylalanine-Glu-Ala-2-aminohexanoic acid-Gly-NH2 pH 4.7, 25°C, wild-type enzyme equine infectious anemia virus