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Literature summary for 3.4.23.B2 extracted from
- Crystallization and biochemical characterisation of simian immunodeficiency virus proteinase mutant (1994), Biochem. Soc. Trans., 22, 55S.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop crystallization of the K69H mutant enzyme with pepstatin A | Simian immunodeficiency virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D25N | totally inactive mutant enzyme, incapable of autoprocessing | Simian immunodeficiency virus |
| K69H | the isoelectric point of the mutant enzyme is pH 7.0 compared to that of the wild-type enzyme of 7.4. The purified mutant enzyme is less soluble at low pH when concentrating sample in preparation for crystallization | Simian immunodeficiency virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pepstatin A | - |
Simian immunodeficiency virus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Simian immunodeficiency virus | - |
wild-type and mutant enzymes K69H and D25N | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Lys-Ala-Arg-Val-Nle-(NO2)Phe-Glu-Ala-Nle-Gly + H2O | - |
Simian immunodeficiency virus | Lys-Ala-Arg-Val-Nle + (NO2)Phe-Glu-Ala-Nle-Gly | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Simian immunodeficiency virus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Simian immunodeficiency virus | mutant enzyme K69H | - |
7 |
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