Cloned (Comment) | Organism |
---|---|
wild-type and histidine-tagged mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N are expressed in Escherichia coli M15 pDM1 | Rous sarcoma virus |
Crystallization (Comment) | Organism |
---|---|
the multiple substitution allow the protease to cleave four HIV-1 peptide substrates at initial rates more than 100fold greater than wild-type enzyme | Rous sarcoma virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
PPAVSLAMTMRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
0.111 | - |
PARVLFLDGRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
0.132 | - |
PATIMMORERR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
0.283 | - |
PGNFLQSRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
0.366 | - |
PARVLAEAMRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rous sarcoma virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant linked-dimer RSV protease | Rous sarcoma virus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | recombinant enzyme is purified from inclusion bodies in Escherichia coli | Rous sarcoma virus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is a homodimer which acts symmetrically in substrate selection with each enzyme subunit being capable of recognizing both halves of a peptide substrate equally | Rous sarcoma virus | ? | - |
? | |
PARVLAEAMRR + H2O | reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | PARVL + AEAMRR | - |
? | |
PARVLFLDGRR + H2O | reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | PARVL + FLDGRR | - |
? | |
PATIMMORERR + H2O | reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | PATIM + MORERR | - |
? | |
PGNFLQSRR + H2O | reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | PGNF + LQSRR | - |
? | |
PPAVSLAMTMRR + H2O | reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | PPAVS + LAMTMRR | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.283 | - |
PGNFLQSRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
0.717 | - |
PPAVSLAMTMRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
1.63 | - |
PARVLAEAMRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
2.98 | - |
PATIMMORERR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus | |
8.2 | - |
PARVLFLDGRR | pH 5.9, 37°C, reaction with mutant enzyme S38T/I42D/I44V/M73V/A100L/L104T/R105P/G106V/S107N | Rous sarcoma virus |