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Literature summary for 3.4.23.52 extracted from

  • Nair, D.; Jarrell, K.
    Pilin processing follows a different temporal route than that of archaellins in Methanococcus maripaludis (2015), Life, 5, 85-101 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene eppA, recombinant expression of C-terminally FLAG- or His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3) Methanococcus maripaludis
gene flak, recombinant expression of C-terminally FLAG- or His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3) Methanococcus maripaludis

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutant strains DELTAeppA, DELTAeppADELTAaglB, and DELTAflakDELTAeppA. AglB is the oligosaccharyltransferase responsible for the terminal step in the N-linked glycosylation pathway. In the DELTAeppA mutant where the signal peptide is not be removed from the pilins, the proteins EpdE and EpdD migrate faster than the same proteins in wild-type cells, despite the fact the proteins are larger due to the additional signal peptide in the DELTAeppA strain. In the DELTAaglBDELTAeppA double mutant, the electrophoretic mobility of EpdE-FLAG and EpdD-FLAG is the same as it was in the DELTAeppA strain Methanococcus maripaludis
additional information construction of deletion mutant strains DELTAflak, DELTAflakDELTAaglB, and DELTAflakDELTAeppA. AglB is the oligosaccharyltransferase responsible for the terminal step in the N-linked glycosylation pathway Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prearchaellin + H2O Methanococcus maripaludis
-
archaellin + ?
-
?
prearchaellin + H2O Methanococcus maripaludis Mm900
-
archaellin + ?
-
?
prepilin EpdA + H2O Methanococcus maripaludis cleavage sequence of pilins EpdA signal peptide is MFKRFNRG-QISFE pilin EpdA + EpdA signal peptide
-
?
prepilin EpdA + H2O Methanococcus maripaludis Mm900 cleavage sequence of pilins EpdA signal peptide is MFKRFNRG-QISFE pilin EpdA + EpdA signal peptide
-
?
prepilin EpdB + H2O Methanococcus maripaludis cleavage sequence of pilins EpdB signal peptide is MSKG-QVSVE pilin EpdB + EpdB signal peptide
-
?
prepilin EpdB + H2O Methanococcus maripaludis Mm900 cleavage sequence of pilins EpdB signal peptide is MSKG-QVSVE pilin EpdB + EpdB signal peptide
-
?
prepilin EpdC + H2O Methanococcus maripaludis cleavage sequence of pilins EpdC signal peptide is MIKMLQLPFNKKG-QVSFD pilin EpdC + EpdC signal peptide
-
?
prepilin EpdC + H2O Methanococcus maripaludis Mm900 cleavage sequence of pilins EpdC signal peptide is MIKMLQLPFNKKG-QVSFD pilin EpdC + EpdC signal peptide
-
?
prepilin EpdD + H2O Methanococcus maripaludis cleavage sequence of pilins EpdD signal peptide is MSVALKKFFSKRG-QLSLE pilin EpdD + EpdD signal peptide
-
?
prepilin EpdD + H2O Methanococcus maripaludis Mm900 cleavage sequence of pilins EpdD signal peptide is MSVALKKFFSKRG-QLSLE pilin EpdD + EpdD signal peptide
-
?
prepilin EpdE + H2O Methanococcus maripaludis cleavage sequence of pilins EpdE signal peptide is MKFLEKLTSKKG-QIAME pilin EpdE + EpdE signal peptide
-
?

Organism

Organism UniProt Comment Textmining
Methanococcus maripaludis
-
-
-
Methanococcus maripaludis Mm900
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein EpdE is a glycoprotein with multiple sites occupied by an N-linked glycan Methanococcus maripaludis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prearchaellin + H2O
-
Methanococcus maripaludis archaellin + ?
-
?
prearchaellin + H2O
-
Methanococcus maripaludis Mm900 archaellin + ?
-
?
prepilin EpdA + H2O cleavage sequence of pilins EpdA signal peptide is MFKRFNRG-QISFE Methanococcus maripaludis pilin EpdA + EpdA signal peptide
-
?
prepilin EpdA + H2O cleavage sequence of pilins EpdA signal peptide is MFKRFNRG-QISFE Methanococcus maripaludis Mm900 pilin EpdA + EpdA signal peptide
-
?
prepilin EpdB + H2O cleavage sequence of pilins EpdB signal peptide is MSKG-QVSVE Methanococcus maripaludis pilin EpdB + EpdB signal peptide
-
?
prepilin EpdB + H2O cleavage sequence of pilins EpdB signal peptide is MSKG-QVSVE Methanococcus maripaludis Mm900 pilin EpdB + EpdB signal peptide
-
?
prepilin EpdC + H2O cleavage sequence of pilins EpdC signal peptide is MIKMLQLPFNKKG-QVSFD Methanococcus maripaludis pilin EpdC + EpdC signal peptide
-
?
prepilin EpdC + H2O cleavage sequence of pilins EpdC signal peptide is MIKMLQLPFNKKG-QVSFD Methanococcus maripaludis Mm900 pilin EpdC + EpdC signal peptide
-
?
prepilin EpdD + H2O cleavage sequence of pilins EpdD signal peptide is MSVALKKFFSKRG-QLSLE Methanococcus maripaludis pilin EpdD + EpdD signal peptide
-
?
prepilin EpdD + H2O cleavage sequence of pilins EpdD signal peptide is MSVALKKFFSKRG-QLSLE Methanococcus maripaludis Mm900 pilin EpdD + EpdD signal peptide
-
?
prepilin EpdE + H2O cleavage sequence of pilins EpdE signal peptide is MKFLEKLTSKKG-QIAME Methanococcus maripaludis pilin EpdE + EpdE signal peptide
-
?

Subunits

Subunits Comment Organism
? x * 15000-17000, recombinant EpdE from purified pili, SDS-PAGE Methanococcus maripaludis

Synonyms

Synonyms Comment Organism
EppA
-
Methanococcus maripaludis
FlaK
-
Methanococcus maripaludis
peptidase-like enzyme
-
Methanococcus maripaludis
Prepilin peptidase
-
Methanococcus maripaludis
signal peptidase III
-
Methanococcus maripaludis

General Information

General Information Comment Organism
evolution FlaK can only process archaellins and EppA only pilins Methanococcus maripaludis
evolution in Methanococcus maripaludis, the pilins have their own dedicated prepilin peptidase, EppA, which cleaves the signal peptide from pilins but not archaellins. This is in contrast to other studied Archaea which have a single prepilin peptidase-like enzyme, PibD, that cleaves all type IV pilin-like proteins, including archaellins, pilins and sugar binding proteins. Pilins in the organism are encoded by five genes, epdA-E. FlaK can only process archaellins and EppA only pilins Methanococcus maripaludis
additional information in Methanococcus maripaludis, the archaella are composed of three structural glycoproteins (the archaellins, FlaB1, FlaB2 and FlaB3) that are all modified at multiple positions with an N-linked tetrasaccharide Methanococcus maripaludis
physiological function Methanococcus maripaludis has two different surface appendages: type IV-like pili and archaella. Both structures are believed to be assembled using a bacterial type IV pilus mechanism. Each structure is composed of multiple subunits, either pilins or archaellins. Both pilins and archaellins are made initially as preproteins with type IV pilin-like signal peptides, which must be removed by a prepilin peptidase-like enzyme. This enzyme is FlaK for archaellins and EppA for pilins. Both archaella and archaeal type IV pili are assembled from structural proteins synthesized initially as preproteins with a class III (type IV pilin-like) signal peptide which is subsequently cleaved by a dedicated prepilin peptidase-like enzyme (signal peptidase III) Methanococcus maripaludis
physiological function Methanococcus maripaludis has two different surface appendages: type IV-like pili and archaella. Both structures are believed to be assembled using a bacterial type IV pilus mechanism. Each structure is composed of multiple subunits, either pilins or archaellins. Both pilins and archaellins are made initially as preproteins with type IV pilin-like signal peptides, which must be removed by a prepilin peptidase-like enzyme. This enzyme is FlaK for archaellins and EppA for pilins. The Epd pili of Methanococcus maripaludis are less numerous and thinner than archaella. Prepilin peptidase EppA cleaves the signal peptide from pilins but not archaellins Methanococcus maripaludis