Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | wild type enzyme is not affected by 1,2-ethanedithiol dimethanesulfonate and dithiothreitol | Methanococcus maripaludis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Methanococcus maripaludis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 30% (w/v) PEG 300, 50 mM glycine (pH 9.5) and 100 mM NaCl | Methanococcus maripaludis |
Protein Variants | Comment | Organism |
---|---|---|
E25C/I206C | the mutant is proteolytically active | Methanococcus maripaludis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Methanococcus maripaludis | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus maripaludis | A9A677 | - |
- |
Purification (Comment) | Organism |
---|---|
metal-affinity column chromatography and gel filtration | Methanococcus maripaludis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
preflagellin FlaB2 + H2O | - |
Methanococcus maripaludis | flagellin FlaB2 + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FlaK | - |
Methanococcus maripaludis |