Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.52 extracted from

  • Bardy, S.L.; Jarrell, K.F.
    Cleavage of preflagellins by an aspartic acid signal peptidase is essential for flagellation in the archaeon Methanococcus voltae (2003), Mol. Microbiol., 50, 1339-1347.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in BL21(DE3)/pLysS membranes Methanococcus voltae

Protein Variants

Protein Variants Comment Organism
D186A the mutation does not affect the activity towards preflagellin FlaB2 Methanococcus voltae
D18A inactive Methanococcus voltae
D18E the mutation results in an apparently unstable protein Methanococcus voltae
D18N inactive Methanococcus voltae
D190A the mutation does not affect the activity towards preflagellin FlaB2 Methanococcus voltae
D224A the mutation does not affect the activity towards preflagellin FlaB2 Methanococcus voltae
D79A inactive Methanococcus voltae
D79E the mutant restores preflagellin peptidase activity (despite a reduced amount of peptidase produced) Methanococcus voltae
D79N inactive Methanococcus voltae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Methanococcus voltae 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25900
-
x * 25900, calculated from amino acid sequence Methanococcus voltae

Organism

Organism UniProt Comment Textmining
Methanococcus voltae
-
-
-
Methanococcus voltae PS
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
preflagellin FlaB2 + H2O
-
Methanococcus voltae flagellin FlaB2 + ?
-
?
preflagellin FlaB2 + H2O
-
Methanococcus voltae PS flagellin FlaB2 + ?
-
?

Subunits

Subunits Comment Organism
? x * 25900, calculated from amino acid sequence Methanococcus voltae

Synonyms

Synonyms Comment Organism
FlaK
-
Methanococcus voltae
RMVO00262 recombinant enzyme Methanococcus voltae

General Information

General Information Comment Organism
physiological function the enzyme plays an essential role in flagellation Methanococcus voltae