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Literature summary for 3.4.23.50 extracted from
- Human retrovirus K10 retropepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 182-184.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | high ionic strength activates the enzyme | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| sequence determination and analysis, expression of wild-type and C-terminally truncated mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a C-terminally truncated mutant enzyme shows increased affinity for the peptide substrates and increased sensitivity to peptidomimetic inhibitors compared to the wild-type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| indinavir | weak inhibition | Homo sapiens |  |
| pepstatin | weak inhibition | Homo sapiens | |
| ritonavir | weak inhibition | Homo sapiens | |
| saquinavir | weak inhibition | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 1 M, activates | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17500 | - |
2 * 17500, the enzyme is synthesized as monomeric part of the Gag polyprotein or the Gag-Pol polyprotein and autolytically processed to the mature protein dimer, structure overview | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gag polyprotein + H2O | Homo sapiens | the enzyme is synthesized as part of the Gag polyprotein or the Gag-Pol polyprotein and autolytically processed to the mature dimer | ? | - |
? | |
| Gag-Pol polyprotein + H2O | Homo sapiens | the enzyme is synthesized as part of the Gag polyprotein or the Gag-Pol polyprotein and autolytically processed to the mature dimer | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
HERV K10, belongs to the human endogenous retrovirus K group | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the enzyme is synthesized as monomeric part of the Gag polyprotein or the Gag-Pol polyprotein and autolytically processed to the mature protein dimer, autoprocessing at the N-terminal sequence Lys-Ala-Ala-Tyr-Trp-Ala-Ser-Gln | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and C-terminally truncated mutant enzymes from Escherichia coli by ammonium sulfate fractionation, cation and anion exchange chromatography, and hydrophobic interaction chromatography, or recombinant C-terminally truncated mutant enzyme from inclusion bodies by dialysis and pepstatin A affinity chromatography followed by autoprocession to active, soluble enzme | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Processing at the authentic HIV-1 PR recognition site and release of the mature p17 matrix and the p24 capsid protein, as a result of the cleavage of the -SQNY-/-PIVQ- cleavage site. | the enzyme contains the aspartic protease triad Asp-Thr-Gly in the catalytic site | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminobenzoyl-ATHQVY-(4-nitro)FVRKA + H2O | - |
Homo sapiens | 2-aminobenzoyl-ATHQVY + (4-nitro)FVRKA | - |
? | |
| Gag polyprotein + H2O | the enzyme is synthesized as part of the Gag polyprotein or the Gag-Pol polyprotein and autolytically processed to the mature dimer | Homo sapiens | ? | - |
? | |
| Gag-Pol polyprotein + H2O | the enzyme is synthesized as part of the Gag polyprotein or the Gag-Pol polyprotein and autolytically processed to the mature dimer | Homo sapiens | ? | - |
? | |
| KARVY-(4-nitro)FEA-Nle-NH2 + H2O | - |
Homo sapiens | KARVY + (4-nitro)FEA-Nle-NH2 | - |
? | |
| additional information | the C-terminal 13-amino-acid extension of the enzyme might play a regulatory role | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 17500, the enzyme is synthesized as monomeric part of the Gag polyprotein or the Gag-Pol polyprotein and autolytically processed to the mature protein dimer, structure overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HERV K10 retropepsin | - |
Homo sapiens |
| human retrovirus K10 retropepsin | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
C-terminally truncated mutant enzyme, substrate KARVYF-NO2-EA-Nle-NH2 | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | room temperature, prolonged incubation, 60% remaining activity in presence or absence of 1 M NaCl, no autolysis | Homo sapiens |
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