Cloned (Comment) | Organism |
---|---|
213 amino acids of the 3'-end of the HERV-K protease open reading frame are expressed in Escherichia coli. Autocatalytic cleavage of the expressed polypeptide results in a catalytically active 18200 Da protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ABT538 | - |
Homo sapiens | |
KNI227 | - |
Homo sapiens | |
KNI272 | - |
Homo sapiens | |
L735,524 | - |
Homo sapiens | |
additional information | the enzyme is highly resistant to all the HIV-1 PR inhibitors tested, including L-735,524, Ro31-8959, and ABT 538 | Homo sapiens | |
pepstatin A | - |
Homo sapiens | |
Ro31-8624 | - |
Homo sapiens | |
Ro31-8959 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Lys-Ala-Arg-Val-Tyr-Phe(NO2)-Glu-Ala-Nle-NH2 | pH 4.5, 11600 Da core domain | Homo sapiens | |
0.0103 | - |
Lys-Ala-Arg-Val-Tyr-Phe(NO2)-Glu-Ala-Nle-NH2 | pH 4.5, 18200 Da enzyme form | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16470 | - |
avarage molecular weight under near physiological conditions, sedimentation equilibrium analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
18200 Da enzyme form and 11600 Da core domain | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
HIV-1 matrix-capsid polyprotein + H2O | processing at the authentic HIV-1 PR recognition site | Homo sapiens | ? | - |
? | |
Lys-Ala-Arg-Val-Tyr-Phe(NO2)-Glu-Ala-Nle-NH2 + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the 11600 Da core domain exists as a dimer at pH 7.0 | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
Lys-Ala-Arg-Val-Tyr-Phe(NO2)-Glu-Ala-Nle-NH2 | pH 4.5, 18200 Da enzyme form | Homo sapiens | |
2.1 | - |
Lys-Ala-Arg-Val-Tyr-Phe(NO2)-Glu-Ala-Nle-NH2 | pH 4.5, 11600 Da core domain | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | 11600 Da core domain | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
0.0000046 | - |
KNI227 | pH 4.5, 11600 Da core domain | Homo sapiens | |
0.000015 | - |
KNI227 | pH 4.5, 18200 Da enzyme form | Homo sapiens | |
0.00006 | - |
ABT538 | pH 4.5, 11600 Da core domain | Homo sapiens | |
0.00012 | - |
KNI272 | pH 4.5, 11600 Da core domain | Homo sapiens | |
0.00034 | - |
KNI272 | pH 4.5, 18200 Da enzyme form | Homo sapiens |