Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | cathepsin D has neither endogenous lysosomal nor cytosolic inhibitors | Rattus norvegicus | |
pepstatin A | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | procathepsin D is transported from cisterns of the rough endoplasmic reticulum to the Golgi apparatus | Rattus norvegicus | 5794 | - |
lysosome | - |
Rattus norvegicus | 5764 | - |
rough endoplasmic reticulum | cathepsin D is synthesized in the rough endoplasmic reticulum as preprocathepsin D | Rattus norvegicus | 5791 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
1 * 34000 + 1 * 14000, mature cathepsin D | Rattus norvegicus |
34000 | - |
1 * 34000 + 1 * 14000, mature cathepsin D | Rattus norvegicus |
48000 | - |
single chain intermediate cathepsin D | Rattus norvegicus |
52000 | - |
pro-cathepsin D | Rattus norvegicus |
54000 | - |
prepro-cathepsin D | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Albumin + H2O | Rattus norvegicus | 15% cleavage at pH 3.0 | ? | - |
? | |
alpha-globulin + H2O | Rattus norvegicus | 12% cleavage at pH 3.0 and 3.5 | ? | - |
? | |
beta-globulin + H2O | Rattus norvegicus | 10% cleavage at pH 3.0 and 3.5 | ? | - |
? | |
casein + H2O | Rattus norvegicus | 20% cleavage at pH 3.5 | ? | - |
? | |
Fibrin + H2O | Rattus norvegicus | 12% cleavage at pH 3.0 | ? | - |
? | |
Hemoglobin + H2O | Rattus norvegicus | 100% cleavage at pH 3.0 | ? | - |
? | |
additional information | Rattus norvegicus | does not cleave gamma-globulin and fibrinogen | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | highest hydrolytic activity | Rattus norvegicus | - |
blood plasma | - |
Rattus norvegicus | - |
bone marrow | - |
Rattus norvegicus | - |
cerebellum | - |
Rattus norvegicus | - |
cerebrum | - |
Rattus norvegicus | - |
colon | - |
Rattus norvegicus | - |
duodenum | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
ileum | - |
Rattus norvegicus | - |
jejunum | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
Kupffer cell | Kupffer cells exhibit highest liver cathepsin D activity | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
lymph node | - |
Rattus norvegicus | - |
additional information | very low detection in serum and blood cells | Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
stomach | - |
Rattus norvegicus | - |
thymus | - |
Rattus norvegicus | - |
urinary bladder | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Albumin + H2O | 15% cleavage at pH 3.0 | Rattus norvegicus | ? | - |
? | |
alpha-globulin + H2O | 12% cleavage at pH 3.0 and 3.5 | Rattus norvegicus | ? | - |
? | |
AMCA-Glu-Glu-L-Lys-L-Pro-L-Ile-L-Ser-L-Phe-L-Phe-L-Arg-L-Leu-Gly-L-Lys(biotinyl)-NH2 + H2O | - |
Rattus norvegicus | AMCA-Glu-Glu-L-Lys-L-Pro-L-Ile-L-Ser-L-Phe + L-Phe-L-Arg-L-Leu-Gly-L-Lys(biotinyl)-NH2 | - |
? | |
benzoyl-L-Arg-Gly-L-Phe-L-Phe-L-Leu-4-methoxy-2-naphthylamide + H2O | - |
Rattus norvegicus | ? | - |
? | |
benzoyl-L-Arg-L-Pro-L-Phe-L-Phe-L-Leu-4-methoxy-2-naphthylamide + H2O | - |
Rattus norvegicus | ? | - |
? | |
benzyloxycarbonyl-L-Pro-L-Phe-L-His-L-Leu-L-Leu-L-Val-L-Tyr-L-Ser-2-naphthylamide + H2O | - |
Rattus norvegicus | ? | - |
? | |
beta-globulin + H2O | 10% cleavage at pH 3.0 and 3.5 | Rattus norvegicus | ? | - |
? | |
casein + H2O | 20% cleavage at pH 3.5 | Rattus norvegicus | ? | - |
? | |
D-Phe-L-Ser-L-Phe-L-Phe-L-Ala-L-Ala-4-aminobenzoate + H2O | - |
Rattus norvegicus | D-Phe-L-Ser-L-Phe + L-Phe-L-Ala-L-Ala-4-aminobenzoate | - |
? | |
D-Phe-L-Ser-L-Ser-L-Phe-L-Phe-L-Ala-4-aminobenzoate + H2O | - |
Rattus norvegicus | ? | - |
? | |
Fibrin + H2O | 12% cleavage at pH 3.0 | Rattus norvegicus | ? | - |
? | |
Gly-L-Phe-L-Leu-Gly-D-Phe-L-Leu + H2O | 3.0% cleavage | Rattus norvegicus | Gly-L-Phe-L-Leu-Gly + D-Phe-L-Leu | - |
? | |
Gly-L-Phe-L-Leu-Gly-L-Phe-D-Leu + H2O | 6.0% cleavage | Rattus norvegicus | Gly-L-Phe-L-Leu-Gly + L-Phe-D-Leu | - |
? | |
Gly-L-Phe-L-Leu-Gly-L-Phe-L-Leu + H2O | 100% cleavage | Rattus norvegicus | Gly-L-Phe-L-Leu-Gly + L-Phe-L-Leu | - |
? | |
Hemoglobin + H2O | 100% cleavage at pH 3.0 | Rattus norvegicus | ? | - |
? | |
L-Asp-L-Arg-L-Val-L-Tyr-L-Ile-L-His-L-Pro-L-Phe-L-His-L-Leu-L-Leu-L-Val-L-Tyr-L-Ser-OH + H2O | - |
Rattus norvegicus | ? | - |
? | |
L-Asp-L-Arg-L-Val-L-Tyr-L-Ile-L-His-L-Pro-L-Phe-L-His-L-Leu-L-Val-L-Ile-L-His-OH + H2O | - |
Rattus norvegicus | ? | - |
? | |
L-Asp-L-Val-L-Arg-L-Tyr-L-Ile-L-His-L-Pro-L-Phe-L-His-L-Leu-L-Leu-L-Val-L-Tyr-L-Ser-OH + H2O | - |
Rattus norvegicus | ? | - |
? | |
L-Phe-L-Ala-L-Ala-L-Phe(NO2)-L-Phe-L-Val-L-Leu-4-hydroxymethyl(pyridine) + H2O | - |
Rattus norvegicus | L-Phe-L-Ala-L-Ala-L-Phe(NO2) + L-Phe-L-Val-L-Leu-4-hydroxymethyl(pyridine) | - |
? | |
additional information | does not cleave gamma-globulin and fibrinogen | Rattus norvegicus | ? | - |
? | |
additional information | does not cleave Gly-D-Phe-L-Leu-Gly-L-Phe-L-Leu, Gly-D-Phe-L-Leu-Gly-D-Phe-L-Leu, and cyclic Gly-L-Phe-L-Leu-Gly-L-Phe-L-Leu | Rattus norvegicus | ? | - |
? | |
N-acetyl-Gly-L-Phe-L-Leu-Gly-L-Phe-OH + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 34000 + 1 * 14000, mature cathepsin D | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
CTSD | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | at 20°C: about 70% of maximum activity, at 50°C: about 30% of maximum activity | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | completely stable between 20 and 40°C, thereafter activity decreases continuously, 20% loss of activity at 50°C, 80% loss of activity at 60°C, complete inactivation at 65°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
complexification of hemoglobin with haptoglobin reduces the optimum pH from 3.0 to 2.8 for degradation and simultaneously causes a 50% decrease in degradation rate 50% | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | at pH 3.0: about 25% of maximum activity, at pH 5.0: about 45% of maximum activity, beta-endorphin hydrolysis | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 8 | completely stable at pH 4.5-5.5, 90% loss of activity at pH 2.5, about 40% loss of activity at pH 3.0, about 5% loss of activity at pH 3.5-4.0, about 10% loss of activity at pH 6.0, about 30% loss of activity at pH 7.0, about 50% loss of activity at pH 7.5, about 80% loss of activity at pH 8.0 | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00001 | - |
IC50 less than 0.00001 mM, pH and temperature not specified in the publication | Rattus norvegicus | pepstatin A |