Literature summary for 3.4.23.5 extracted from

Demoz, M.; Castino, R.; Follo, C.; Hasilik, A.; Sloane, B.F.; Isidoro, C.
High yield synthesis and characterization of phosphorylated recombinant human procathepsin D expressed in mammalian cells (2006), Protein Expr. Purif., 45, 157-167.

Cloned(Commentary)

Cloned (Comment)	Organism
a vaccinia virus expression system is used for the production of recombinant human cathepsin D in human cell lines MCF7 (breast cancer), HepG2 (hepatocarcinoma) and HeLa (cervix cancer) and non-human cell line BSC1 (simian kidney epithelial). For generation of a recombinant vaccinia virus the full-length cathepsin D cDNA comprising the ATG codon is inserted downstream of a synthetic vaccinia late promoter into the vector pMJ601 which also contains the Escherichia coli LacZ gene. This expression cassette is flanked by segments of the vaccinia thymidine kinase (TK) gene that allows homologous recombination with the wild-type virus	Homo sapiens

Cloned (Comment)

Organism

a vaccinia virus expression system is used for the production of recombinant human cathepsin D in human cell lines MCF7 (breast cancer), HepG2 (hepatocarcinoma) and HeLa (cervix cancer) and non-human cell line BSC1 (simian kidney epithelial). For generation of a recombinant vaccinia virus the full-length cathepsin D cDNA comprising the ATG codon is inserted downstream of a synthetic vaccinia late promoter into the vector pMJ601 which also contains the Escherichia coli LacZ gene. This expression cassette is flanked by segments of the vaccinia thymidine kinase (TK) gene that allows homologous recombination with the wild-type virus

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
pepstatin A	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
13000	-	double chain form	Homo sapiens
31000	-	double chain form	Homo sapiens
48000	-	intermediate	Homo sapiens
53000	-	precursor (proCDrec)	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	proCDrec is largely secreted into the culture medium, although it contains high-mannose oligosaccharides with uncovered mannose-6-phosphate residues	Homo sapiens
proteolytic modification	intracellular proCDrec is processed into the 48 kDa intermediate single-chain and the 31 plus 13 kDa double-chain form. Processing is slower than in normal cells	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
using pepstatin A-chromatography	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	during incubation at acid pH the purified proCDrec undergoes autocatalytic processing and acquires pepstatin A-sensitive enzyme activity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aminomethylcoumarine-conjugated hemoglobin + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
cathepsin D	-	Homo sapiens
proCDrec	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.6	5	assay at	Homo sapiens