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Literature summary for 3.4.23.5 extracted from

  • Goldman-Levkovitz, S.; Rimon, A.; Rimon, S.
    Purification properties and specificity of cathepsin D from Cyprinus carpio (1995), Comp. Biochem. Physiol. B, 112, 147-151.
    View publication on PubMed
Search for more literature for 3.4.23.5

Inhibitors

Inhibitors Comment Organism Structure
pepstatin 0.001 mM, complete inhibition Cyprinus carpio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Cyprinus carpio

Organism

Organism UniProt Comment Textmining
Cyprinus carpio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cyprinus carpio

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Cyprinus carpio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cyprinus carpio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Albumin + H2O
-
 Cyprinus carpio ?
-
 ?
Hemoglobin + H2O
-
 Cyprinus carpio ?
-
 ?
insulin + H2O 56% cleavage at Tyr16-Leu17, 40% at Phe25-Tyr26. The minor points of cleavage are at Leu15-Tyr16, Phe24-Phe25, Gly23-Phe24, Leu11-Val12, Ala14-Leu15 and Gln4-His5 Cyprinus carpio ?
-
 ?
insulin + H2O beta-chain of oxidized insulin Cyprinus carpio ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Cyprinus carpio

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43 55
-
 Cyprinus carpio

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 10 min, at pH 3.5 or at pH 7.5, stable Cyprinus carpio
43
-
 10 min, stable at pH 3.5, 40% loss of activity at pH 7.5 Cyprinus carpio
48.5
-
 10 min, stable at pH 3.5, 81% loss of activity at pH 7.5 Cyprinus carpio
54
-
 10 min, 13% loss of activity at pH 3.5, complete loss of activity at pH 7.5 Cyprinus carpio
60
-
 10 min, 35% loss of activity at pH 3.5, complete loss of activity at pH 7.5 Cyprinus carpio
66
-
 10 min, complete loss of activity at pH 3.5 and at pH 7.5 Cyprinus carpio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7 3.7 hemoglobin Cyprinus carpio
3
-
 albumin Cyprinus carpio

pH Range

pH Minimum pH Maximum Comment Organism
2 5 pH 2.0: about 55% of maximal activity, pH 5.0: about40% of maximal activity, with albumin as substrate Cyprinus carpio
2 4.5 pH 2.0: about 35% of maximal activity, pH 4.5: about 45% of maximal activity, hemoglobin as substrate Cyprinus carpio