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Literature summary for 3.4.23.48 extracted from

  • Dentovskaya, S.V.; Platonov, M.E.; Svetoch, T.E.; Kopylov, P.K.; Kombarova, T.I.; Ivanov, S.A.; Shaikhutdinova, R.Z.; Kolombet, L.V.; Chauhan, S.; Ablamunits, V.G.; Motin, V.L.; Uversky, V.N.; Anisimov, A.P.
    Two isoforms of Yersinia pestis plasminogen activator Pla intraspecies distribution, intrinsic disorder propensity, and contribution to virulence (2016), PLoS ONE, 11, e0168089 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.48

Cloned(Commentary)

Cloned (Comment) Organism
Pla is encoded by the 9.5 kb Yersinia pestis plasmid pPCP, sequence comparisons Yersinia pestis subsp. pestis
Pla is encoded by the 9.5 kb Yersinia pestis plasmid pPCP, sequencing of pla genes from 118 Yersinia pestis subsp. microtus isolates, sequence comparisons Yersinia pestis subsp. microtus

Protein Variants

Protein Variants Comment Organism
additional information the Pla I259T modification is not required to cause pneumonic plague in the murine intranasal model Yersinia pestis subsp. pestis

Inhibitors

Inhibitors Comment Organism Structure
plasminogen activator inhibitor 1 PAI-1 Yersinia pestis subsp. microtus
plasminogen activator inhibitor 1 PAI-1 Yersinia pestis subsp. pestis

Organism

Organism UniProt Comment Textmining
Yersinia pestis subsp. microtus A0A141BQM9 SNP-types 0.PE3 and 0.PE4
-
Yersinia pestis subsp. microtus 91001 A0A141BQM9 SNP-types 0.PE3 and 0.PE4
-
Yersinia pestis subsp. pestis P17811 T259 isotype
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme performs autoproteolysis Yersinia pestis subsp. microtus
proteolytic modification the enzyme performs autoproteolysis Yersinia pestis subsp. pestis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(DABCYL)-Arg-Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 + H2O
-
 Yersinia pestis subsp. microtus (DABCYL)-Arg + Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2
-
 ?
(DABCYL)-Arg-Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 + H2O
-
 Yersinia pestis subsp. pestis (DABCYL)-Arg + Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2
-
 ?
(DABCYL)-Arg-Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2 + H2O
-
 Yersinia pestis subsp. microtus 91001 (DABCYL)-Arg + Arg-Ile-Asn-Arg-Glu-(EDANS)-NH2
-
 ?
additional information the enzyme performs autoproteolysis Yersinia pestis subsp. microtus ?
-
 ?
additional information the enzyme performs autoproteolysis Yersinia pestis subsp. pestis ?
-
 ?
additional information the enzyme performs autoproteolysis Yersinia pestis subsp. microtus 91001 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34100, SDS-PAGE, x * 32600, about, unprocessed enzyme, sequence calculation, x * 29600, about, processed enzyme, sequence calculation Yersinia pestis subsp. pestis
? x * 36900, SDS-PAGE, x * 32600, about, unprocessed enzyme, sequence calculation, x * 29600, about, processed enzyme, sequence calculation Yersinia pestis subsp. microtus
More the Pla peptide has an atypical mobility on the protein gel Yersinia pestis subsp. microtus
More the Pla peptide has an atypical mobility on the protein gel Yersinia pestis subsp. pestis

Synonyms

Synonyms Comment Organism
coagulase
-
 Yersinia pestis subsp. microtus
coagulase
-
 Yersinia pestis subsp. pestis
fibrinolysin
-
 Yersinia pestis subsp. microtus
fibrinolysin
-
 Yersinia pestis subsp. pestis
Pla
-
 Yersinia pestis subsp. microtus
Pla
-
 Yersinia pestis subsp. pestis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Yersinia pestis subsp. microtus
37
-
 assay at Yersinia pestis subsp. pestis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Yersinia pestis subsp. microtus
7.4
-
 assay at Yersinia pestis subsp. pestis

General Information

General Information Comment Organism
evolution the modern isoform of Pla (T259) with an increased protease activity is found in Yersinia pestis subsp. pestis strains that are highly virulent for humans. The pathogenicity factor is absent in representatives of the Yersinia pestis subsp. microtus bv. caucasica/SNP-type 0.PE2, while an ancestral Pla isoform (I259) with characteristics similar to the properties of omptins from less virulent enterobacteria is found in three representatives of Yersinia pestis subsp. microtus (SNP-types 0.PE3 and 0.PE4), which are, as a rule, avirulent to guinea pigs and humans. Distribution of Pla isoforms among natural isolates of Yersinia pestis of different origin, overview Yersinia pestis subsp. pestis
evolution the pathogenicity factor is absent in representatives of the Yersinia pestis subsp. microtus bv. caucasica/SNP-type 0.PE2, while an ancestral Pla isoform (I259) with characteristics similar to the properties of omptins from less virulent enterobacteria is found in three representatives of Yersinia pestis subsp. microtus (SNP-types 0.PE3 and 0.PE4), which are, as a rule, avirulent to guinea pigs and humans. The modern isoform of Pla (T259) with an increased protease activity is found in Yersinia pestis subsp. pestis strains that are highly virulent for humans. Sequencing of pla genes from 118 Yersinia pestis subsp. microtus isolates reveals the absence of this gene in the strains belonging to bv. caucasica. All remaining isolates of Yersinia pestis subsp. microtus contain the ancestral Pla isoform (I259). Distribution of Pla isoforms among natural isolates of Yersinia pestis of different origin, overview Yersinia pestis subsp. microtus
additional information the primary action of Pla is to protect bacteria from destruction rather than to alter the tissue environment to favor Yersinia pestis propagation in the host. Analysis of intraspecies distribution, intrinsic disorder propensity, and contribution to virulence of the two isoforms of Yersinia pestis plasminogen activator Pla, overview. Survival curves of the endemic I259 Pla+ strains are similar to the parent Pla-negative variants, but significant difference in mean time to death post infection between the Pla- strains and their I259 Pla+ variants can be seen only in the isogenic set of Yersinia pestis subsp. pestis strains. An essential role for the outer membrane protease Pla evolution in Yersinia pestis bubonic infection exacerbation is suggested that is necessary for intensification of epidemic process from endemic natural focality with sporadic cases in humans to rapidly expanding epizootics followed by human epidemic outbreaks, local epidemics or even pandemics. Pla expression is associated with a marked ability to colonize the viscera and thus cause lethal infection upon administration by peripheral routes of infection, such as intradermal or subcutaneous. The Pla activity is not required to initiate lethal disease by intravenous injection, which provides immediate access to fixed macrophages lining the capillary beds of the liver and spleen. Virulence study of Yersinia pestis expressing different isoforms of Pla in a bubonic plague model Yersinia pestis subsp. microtus
additional information the primary action of Pla is to protect bacteria from destruction rather than to alter the tissue environment to favor Yersinia pestis propagation in the host. Analysis of intraspecies distribution, intrinsic disorder propensity, and contribution to virulence of the two isoforms of Yersinia pestis plasminogen activator Pla, overview. Survival curves of the endemic I259 Pla+ strains are similar to the parent Pla-negative variants, but significant difference in mean time to death post infection between the Pla- strains and their I259 Pla+ variants can be seen only in the isogenic set of Yersinia pestis subsp. pestis strains. Pla expression is associated with a marked ability to colonize the viscera and thus cause lethal infection upon administration by peripheral routes of infection, such as intradermal or subcutaneous. The Pla activity is not required to initiate lethal disease by intravenous injection, which provides immediate access to fixed macrophages lining the capillary beds of the liver and spleen. Virulence study of Yersinia pestis expressing different isoforms of Pla in a bubonic plague model Yersinia pestis subsp. pestis