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Literature summary for 3.4.23.47 extracted from

  • Rose, J.R.; Salto, R.; Craik, C.S.
    Regulation of autoproteolysis of the HIV-1 and HIV-2 proteases with engineered amino acid substitutions (1993), J. Biol. Chem., 268, 11939-11945.
    View publication on PubMed
Search for more literature for 3.4.23.47

Application

Application Comment Organism
medicine potential target for chemotherapy of virus infection and associated diseases, essential for maturation of infectious virions, development of drugs against the protease should be effective against HIV-2 Human immunodeficiency virus 2

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli JM-101, F' and X90 Human immunodeficiency virus 2

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized by hanging drop method in complex with a reduced amide inhibitor, BI-LA-398 Human immunodeficiency virus 2

General Stability

General Stability Organism
activity is lost during purification and storage Human immunodeficiency virus 2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.74
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C Human immunodeficiency virus 2
0.95
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C, mutant K7Q Human immunodeficiency virus 2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GAG precursor protein of HIV-2 + H2O Human immunodeficiency virus 2
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 2
-
 mutant K7Q
-
Human immunodeficiency virus 2
-
 human immunodeficiency virus type 2
-

Purification (Commentary)

Purification (Comment) Organism
mutant K7Q, autoproteolysis Human immunodeficiency virus 2
recombinant enzyme Human immunodeficiency virus 2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GAG precursor protein of HIV-2 + H2O
-
 Human immunodeficiency virus 2 ?
-
 ?
additional information cleavage sites identified by amino-terminal sequence analysis are F3-S4, Y14-I15, G35-I36, G52-F53, M76-T77, L90-T91 Human immunodeficiency virus 2 ?
-
 ?
NH2-ATLNFPSPW-COOH + H2O
-
 Human immunodeficiency virus 2 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C Human immunodeficiency virus 2
4.33
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C, mutant K7Q Human immunodeficiency virus 2