Literature summary for 3.4.23.46 extracted from

Sardana, V.; Xu, B.; Zugay-Murphy, J.; Chen, Z.; Sardana, M.; Darke, P.L.; Munshi, S.; Kuo, L.C.
A general procedure for the purification of human beta-secretase expressed in Escherichia coli (2004), Protein Expr. Purif., 34, 190-196.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli with different N-terminal extensions and without the C-terminus transmembrane domain. Each of the proteins is expressed in inclusion bodies	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.055	-	7-methoxycoumarin-4-yl-SEVNLDAEFK-2,4-dinitrophenyl-RR	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P56817	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-methoxycoumarin-4-yl-SEVNLDAEFK-2,4-dinitrophenyl-RR + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
BACE1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.155	-	7-methoxycoumarin-4-yl-SEVNLDAEFK-2,4-dinitrophenyl-RR	-	Homo sapiens

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Release 2023.1 (January 2023)