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Literature summary for 3.4.23.43 extracted from
- Type IV prepilin peptidase (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 204-208.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
prepilin type IV | - |
Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral protein | Bacillus subtilis | 16020 | - |
| membrane | integral protein | Escherichia coli | 16020 | - |
| membrane | integral protein | Pseudomonas aeruginosa | 16020 | - |
| membrane | integral protein | Neisseria gonorrhoeae | 16020 | - |
| membrane | integral protein | Klebsiella pneumoniae | 16020 | - |
| membrane | integral protein | Dickeya chrysanthemi | 16020 | - |
| membrane | integral protein | Xanthomonas campestris | 16020 | - |
| membrane | integral protein | Vibrio cholerae serotype O1 | 16020 | - |
| membrane | integral protein | Vibrio vulnificus | 16020 | - |
| membrane | integral protein | Legionella pneumoniae | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Klebsiella pneumoniae | the enzyme is involved in secretion of pullulanase | ? | - |
? |  |
| additional information | Legionella pneumoniae | the enzyme is involved in type II secretion pathway and intracellular survival | ? | - |
? | |
| prepilin type IV + H2O | Bacillus subtilis | prepilin maturation is required for extracellular protein secretion, and competence state and DNA uptake, overview | pilin type IV + leader peptide | - |
? | |
| prepilin type IV + H2O | Escherichia coli | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Pseudomonas aeruginosa | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Neisseria gonorrhoeae | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Klebsiella pneumoniae | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Vibrio cholerae serotype O1 | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Legionella pneumoniae | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Dickeya chrysanthemi | prepilin maturation is required for extracellular protein secretion of degradative enzymes and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Xanthomonas campestris | prepilin maturation is required for extracellular protein secretion of degradative enzymes and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | Vibrio vulnificus | prepilin maturation is required for extracellular protein secretion of degradative enzymes and DNA uptake, overview | pilin type IV + prepilin type IV leader peptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
gene comC | - |
| Dickeya chrysanthemi | - |
gene outO | - |
| Escherichia coli | - |
genes gspO and pppA | - |
| Klebsiella pneumoniae | - |
- |
- |
| Legionella pneumoniae | - |
gene pilD | - |
| Neisseria gonorrhoeae | - |
gene pilD | - |
| Pseudomonas aeruginosa | - |
gene pilD | - |
| Vibrio cholerae serotype O1 | - |
gene tcpJ | - |
| Vibrio vulnificus | - |
gene vvpD | - |
| Xanthomonas campestris | - |
gene xpsO | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from membrane by detergent solubilization and immunoaffinity chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is involved in secretion of pullulanase | Klebsiella pneumoniae | ? | - |
? | |
| additional information | the enzyme is involved in type II secretion pathway and intracellular survival | Legionella pneumoniae | ? | - |
? | |
| prepilin type IV + H2O | - |
Bacillus subtilis | pilin type IV + leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion, and competence state and DNA uptake, overview | Bacillus subtilis | pilin type IV + leader peptide | - |
? | |
| prepilin type IV + H2O | - |
Neisseria gonorrhoeae | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | - |
Klebsiella pneumoniae | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | - |
Dickeya chrysanthemi | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | - |
Xanthomonas campestris | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | - |
Vibrio vulnificus | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | - |
Legionella pneumoniae | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | Escherichia coli | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | Pseudomonas aeruginosa | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | Neisseria gonorrhoeae | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | Klebsiella pneumoniae | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | Vibrio cholerae serotype O1 | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion and DNA uptake, overview | Legionella pneumoniae | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion of degradative enzymes and DNA uptake, overview | Dickeya chrysanthemi | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion of degradative enzymes and DNA uptake, overview | Xanthomonas campestris | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | prepilin maturation is required for extracellular protein secretion of degradative enzymes and DNA uptake, overview | Vibrio vulnificus | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | the consensus sequence of the cleavage site is Gly-Phe or Gly-Met within Gly-Phe/Met-Thr-Leu-Ile/Leu-Glu | Escherichia coli | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | the consensus sequence of the cleavage site is Gly-Phe or Gly-Met within Gly-Phe/Met-Thr-Leu-Ile/Leu-Glu | Pseudomonas aeruginosa | pilin type IV + prepilin type IV leader peptide | - |
? | |
| prepilin type IV + H2O | the consensus sequence of the cleavage site is Gly-Phe or Gly-Met within Gly-Phe/Met-Thr-Leu-Ile/Leu-Glu | Vibrio cholerae serotype O1 | pilin type IV + prepilin type IV leader peptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Bacillus subtilis |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Escherichia coli |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Pseudomonas aeruginosa |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Neisseria gonorrhoeae |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Klebsiella pneumoniae |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Dickeya chrysanthemi |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Xanthomonas campestris |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Vibrio cholerae serotype O1 |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Vibrio vulnificus |
| More | the cytoplasmic domain contains two pairs of conserved Cys residues important for activity | Legionella pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ComC | - |
Bacillus subtilis |
| More | the enzyme belongs to the A24 peptidase family | Bacillus subtilis |
| More | the enzyme belongs to the A24 peptidase family | Escherichia coli |
| More | the enzyme belongs to the A24 peptidase family | Pseudomonas aeruginosa |
| More | the enzyme belongs to the A24 peptidase family | Neisseria gonorrhoeae |
| More | the enzyme belongs to the A24 peptidase family | Klebsiella pneumoniae |
| More | the enzyme belongs to the A24 peptidase family | Dickeya chrysanthemi |
| More | the enzyme belongs to the A24 peptidase family | Xanthomonas campestris |
| More | the enzyme belongs to the A24 peptidase family | Vibrio cholerae serotype O1 |
| More | the enzyme belongs to the A24 peptidase family | Vibrio vulnificus |
| More | the enzyme belongs to the A24 peptidase family | Legionella pneumoniae |
| PilD | - |
Pseudomonas aeruginosa |
| PilD | - |
Neisseria gonorrhoeae |
| PilD-like protein | - |
Legionella pneumoniae |
| PulO | - |
Klebsiella pneumoniae |
| TcpJ | - |
Vibrio cholerae serotype O1 |
| type IV prepilin peptidase | - |
Pseudomonas aeruginosa |
| type IV prepilin peptidase | - |
Vibrio cholerae serotype O1 |
| type IV prepilin-like peptidase | - |
Bacillus subtilis |
| type IV prepilin-like peptidase | - |
Escherichia coli |
| type IV prepilin-like peptidase | - |
Klebsiella pneumoniae |
| type IV prepilin-like peptidase | - |
Xanthomonas campestris |
| XcpA | - |
Pseudomonas aeruginosa |
| XpsO | - |
Xanthomonas campestris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
prepilin type IV | - |
Pseudomonas aeruginosa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
5.3 |
| Xanthomonas campestris | - |
- |
9.1 |
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Release 2023.1 (January 2023)
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