Literature summary for 3.4.23.41 extracted from

Sohn, M.J.; Oh, D.B.; Kim, E.J.; Cheon, S.A.; Kwon, O.; Kim, J.Y.; Lee, S.Y.; Kang, H.A.
HpYPS1 and HpYPS7 encode functional aspartyl proteases localized at the cell surface in the thermotolerant methylotrophic yeast Hansenula polymorpha (2012), Yeast, 29, 1-16.

Search for more literature for 3.4.23.41

Protein Variants

Protein Variants	Comment	Organism
additional information	heterologous expression of YPS1 completely restores all of the growth defects of the Saccharomyces cerevisiae yps1-deletion strains	Ogataea angusta
additional information	heterologous expression of YPS7 exhibits a limited complementation effect on the Saccharomyces cerevisiae yps7-deletion strain	Ogataea angusta

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	localizes on the cell wall via glycosylphosphatidylinositol anchor	Ogataea angusta	5618	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	1 * 65000-850000, beta-subunit, + 1 * 25000, alpha-subunit. SDS-PAGE, highly glycosylated protein. x * 55000, calculated for mature protein	Ogataea angusta
55000	-	1 * 65000-850000, beta-subunit, + 1 * 25000, alpha-subunit. SDS-PAGE, highly glycosylated protein. x * 55000, calculated for mature protein	Ogataea angusta
57000	-	1 * 150000-200000, SDS-PAGE, highly glycosylated protein. 1 * 57000, calculated for mature protein	Ogataea angusta
65000	100000	PAGE	Ogataea angusta
150000	200000	PAGE	Ogataea angusta

Organism

Organism	UniProt	Comment	Textmining
Ogataea angusta	Q3L7F4	-	-
Ogataea angusta	Q5QT58	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Ogataea angusta
proteolytic modification	sequence contains a N-terminal signal peptide	Ogataea angusta

Purification (Commentary)

Purification (Comment)	Organism
recombinant truncated enzyme	Ogataea angusta

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
human parathyroid hormone + H2O	-	Ogataea angusta	?	proteolysis generates three fragments, one cleavage occurs at dibasic site R25/K26	?
human parathyroid hormone + H2O	-	Ogataea angusta	?	proteolysis generates two fragments, cleavage occurs at dibasic site R25/K26	?

Subunits

Subunits	Comment	Organism
dimer	1 * 65000-850000, beta-subunit, + 1 * 25000, alpha-subunit. SDS-PAGE, highly glycosylated protein. x * 55000, calculated for mature protein	Ogataea angusta
monomer	1 * 150000-200000, SDS-PAGE, highly glycosylated protein. 1 * 57000, calculated for mature protein	Ogataea angusta

Synonyms

Synonyms	Comment	Organism
YPS1	-	Ogataea angusta
YPS7	-	Ogataea angusta

Expression

Organism	Comment	Expression
Ogataea angusta	YPS1 expression is significantly induced only by a subset of stressor agents, such as sodium dodecyl sulfate and tunicamycin	up

General Information

General Information	Comment	Organism
physiological function	deletion of the YPS genes generates only minor influence on the sensitivity to cell wall stress	Ogataea angusta
physiological function	deletion of the YPS genes generates only minor influence on the sensitivity to cell wall stress. The sole disruption of YPS1 is sufficient in eliminating the aberrant proteolytic cleavage of recombinant proteins secreted by Hansenula polymorpha	Ogataea angusta

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Release 2023.1 (January 2023)