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Literature summary for 3.4.23.40 extracted from

  • Sidrach, L.; Garcia-Canovas, F.; Tudela, J.; Rodriguez-Lopez, J.N.
    Purification of cynarases from artichoke (Cynara scolymus L.): enzymatic properties of cynarase A (2005), Phytochemistry, 66, 41-49.
    View publication on PubMed
Search for more literature for 3.4.23.40

Inhibitors

Inhibitors Comment Organism Structure
pepstatin
-
 Cynara cardunculus var. scolymus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 L-Pro-L-Thr-L-Glu-L-Phe(p-NO2)-L-Phe-L-Arg-L-Leu pH 5.0 Cynara cardunculus var. scolymus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11500
-
 1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE Cynara cardunculus var. scolymus
13300
-
 1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE Cynara cardunculus var. scolymus
29700
-
 1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE Cynara cardunculus var. scolymus
32300
-
 1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE Cynara cardunculus var. scolymus
34500
-
 1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE Cynara cardunculus var. scolymus
40800
-
 gel filtration, cynarase A Cynara cardunculus var. scolymus
43400
-
 gel filtration, cynarase B Cynara cardunculus var. scolymus
46000
-
 gel filtration, cynarase C Cynara cardunculus var. scolymus

Organism

Organism UniProt Comment Textmining
Cynara cardunculus var. scolymus
-
 isoforms cynarase A, cynarase B, cynarase C
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isoform cynarase a, cynarase B, and cynarase C. Cynarase A contains N-linked high mannose-type glycans Cynara cardunculus var. scolymus

Purification (Commentary)

Purification (Comment) Organism
from stigma, isoform cynarase a, cynarase B, and cynarase C Cynara cardunculus var. scolymus

Source Tissue

Source Tissue Comment Organism Textmining
stigma
-
 Cynara cardunculus var. scolymus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Pro-L-Thr-L-Glu-L-Phe(p-NO2)-L-Phe-L-Arg-L-Leu + H2O
-
 Cynara cardunculus var. scolymus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 29700 + 1 * 13300, cynarase A, 1 * 34500 + 1 * 11500, cynarase B, 1 * 32300 + 1 * 13300, cynarase C, SDS-PAGE Cynara cardunculus var. scolymus

Synonyms

Synonyms Comment Organism
cynarase A
-
 Cynara cardunculus var. scolymus
cynarase B
-
 Cynara cardunculus var. scolymus
cynarase C
-
 Cynara cardunculus var. scolymus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 isoform cynarase A Cynara cardunculus var. scolymus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, stable Cynara cardunculus var. scolymus
60
-
 rapid inactivation Cynara cardunculus var. scolymus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
144
-
 L-Pro-L-Thr-L-Glu-L-Phe(p-NO2)-L-Phe-L-Arg-L-Leu pH 5.0 Cynara cardunculus var. scolymus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 isoform cynarase A Cynara cardunculus var. scolymus

pH Range

pH Minimum pH Maximum Comment Organism
3
-
 about 40% of maximum activity Cynara cardunculus var. scolymus
7
-
 about 10% of maximum activity Cynara cardunculus var. scolymus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00003
-
 pepstatin pH 5.0 Cynara cardunculus var. scolymus

pI Value

Organism Comment pI Value Maximum pI Value
Cynara cardunculus var. scolymus isoelectric focusing, cynarase A
-
 3.2
Cynara cardunculus var. scolymus isoelectric focusing, cynarase B
-
 3.3
Cynara cardunculus var. scolymus isoelectric focusing, cynarase C
-
 3.4