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Literature summary for 3.4.23.4 extracted from

  • Kageyama, H.; Ueda, H.; Tezuka, T.; Ogasawara, A.; Narita, Y.; Kageyama, T.; Ichinose, M.
    Differences in the P1' substrate specificities of pepsin A and chymosin (2010), J. Biochem., 147, 167-174.
    View publication on PubMed
Search for more literature for 3.4.23.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 AFPLEFFREL pH 4.0, 37°C Bos taurus
0.079
-
 AFPLEFIREL pH 4.0, 37°C Bos taurus
0.18
-
 AFPLEFKREL pH 4.0, 37°C Bos taurus
3.3
-
 AFPLEFEREL pH 4.0, 37°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00794
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AFPLEFEREL + H2O modified peptide substrate based on residues 165-174 of proopiomelanocortin Bos taurus AFPLEF + EREL
-
 ?
AFPLEFFREL + H2O modified peptide substrate based on residues 165-174 of proopiomelanocortin Bos taurus AFPLEF + FREL
-
 ?
AFPLEFIREL + H2O modified peptide substrate based on residues 165-174 of proopiomelanocortin Bos taurus AFPLEF + IREL
-
 ?
AFPLEFKREL + H2O modified peptide substrate based on residues 165-174 of proopiomelanocortin Bos taurus AFPLEF + KREL
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29
-
 AFPLEFKREL pH 4.0, 37°C Bos taurus
290
-
 AFPLEFFREL pH 4.0, 37°C Bos taurus
427
-
 AFPLEFIREL pH 4.0, 37°C Bos taurus
2600
-
 AFPLEFEREL pH 4.0, 37°C Bos taurus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
160
-
 AFPLEFKREL pH 4.0, 37°C Bos taurus
790
-
 AFPLEFEREL pH 4.0, 37°C Bos taurus
5400
-
 AFPLEFIREL pH 4.0, 37°C Bos taurus
7100
-
 AFPLEFFREL pH 4.0, 37°C Bos taurus