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Literature summary for 3.4.23.4 extracted from

  • Starovoitova, V.V.; Velichko, T.I.; Baratova, L.A.; Filippova, I.Y.; Lavrenova, G.I.
    A comparative study of functional properties of calf chymosin and its recombinant forms (2006), Biochemistry (Moscow), 71, 320-324.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Kluyveromyces lactis Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
pepstatin inhibits recombinant and transgenic chymosin less efficiently than the native enzyme Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information transgenic and recombinant chymosin do not hydrolyze substrate Abz-A-A-F-F-A-A-p-nitroanilide, Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine and Abz-A-A-F-F-p-nitroanilide Bos taurus
0.0018
-
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus
0.0018
-
Abz-A-A-F-F-p-nitroanilide hydrolysis by calf chymosin Bos taurus
0.0021
-
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by recombinant chymosin Bos taurus
0.0022
-
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus
0.003
-
Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus
0.0035
-
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by recombinant chymosin Bos taurus
0.004
-
Abz-A-A-F-F-A-A-p-nitroanilide hydrolysis by calf chymosin Bos taurus
0.0056
-
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by recombinant chymosin Bos taurus
0.0056
-
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by transgenic chymosin Bos taurus
0.007
-
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus
0.0078
-
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by transgenic chymosin Bos taurus
0.018
-
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by transgenic chymosin Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography on a bacitracin-Sepharose column Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
abomasum
-
Bos taurus
-
milk transgenic sheep milk Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine + H2O low molecular weight, fluorogenic peptide substrate Bos taurus ?
-
?
Abz-A-A-F-F-A-A-p-nitroanilide + H2O low molecular weight, fluorogenic peptide substrate Bos taurus ?
-
?
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine + H2O low molecular weight, fluorogenic peptide substrate Bos taurus ?
-
?
Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine + H2O low molecular weight, fluorogenic peptide substrate Bos taurus ?
-
?
Abz-A-A-F-F-pnA + H2O low molecular weight, fluorogenic peptide substrate Bos taurus ?
-
?
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine + H2O low molecular weight, fluorogenic peptide substrate Bos taurus ?
-
?
L-S-F-M-A-I-P-NH2 + H2O hepapeptide, a fragment of the native chymosin substrate kappa-casein, is most efficiently cleaved by native calf chymosin and less efficiently by transgenic chymosin and recombinant chymosin Bos taurus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 transgenic chymosin loses activity slightly faster than abomasal enzyme and recombinant enzyme Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by transgenic chymosin Bos taurus
0.06
-
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by transgenic chymosin or recombinat chymosin Bos taurus
0.11
-
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by recombinant chymosin Bos taurus
0.12
-
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by recombinant chymosin Bos taurus
0.14
-
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus
0.16
-
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by transgenic chymosin Bos taurus
0.27
-
Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus
0.48
-
Abz-A-A-F-F-p-nitroanilide hydrolysis by calf chymosin Bos taurus
0.8
-
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus
1.4
-
Abz-A-A-F-F-A-A-p-nitroanilide hydrolysis by calf chymosin Bos taurus
1.6
-
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine hydrolysis by calf chymosin Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 6 under incubation at 37°C for 24 and 48 h, loss of activity at pH 3.5 and pH 7.0, trangenic chymosin and recombinant chymosin are inactivated slightly faster than the native enzyme Bos taurus