Literature summary for 3.4.23.4 extracted from

Pitts, J.E.; Uusitalo, J.M.; Mantafounis, D.; Nugent, P.G.; Quinn, D.D.; Orprayoon, P.; Penttilae, M.E.
Expression and characterisation of chymosin pH optima mutants produced in Trichoderma reesei (1993), J. Biotechnol., 28, 69-83.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
point mutants	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
D304A/T218A	-	Bos taurus
T218A	pH optima shift	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Lys-Pro-Ala-Glu-Phe-Phe(NO2)-Ala-Leu-OH + H2O	-	Bos taurus	Lys-Pro-Ala-Glu-Phe + Phe(NO2)-Ala-Leu	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Bos taurus

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Release 2023.1 (January 2023)