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Literature summary for 3.4.23.39 extracted from

  • Marcoux, J.; Thierry, E.; Vives, C.; Signor, L.; Fieschi, F.; Forest, E.
    Investigating alternative acidic proteases for H/D exchange coupled to mass spectrometry: plasmepsin 2 but not plasmepsin 4 is active under quenching conditions (2010), J. Am. Soc. Mass Spectrom., 21, 76-79.
    View publication on PubMed
Search for more literature for 3.4.23.39

Application

Application Comment Organism
molecular biology plasmepsin 2 but not plasmepsin 4 is a pential tool for hydrogen/deuterium exchange coupled to mass spectroscopy (DXMS) studies Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
PSM2 (residues 78-453) are recombinantly expressed Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 activated form Plasmodium falciparum
43000
-
 precursor form Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plasmepsin 2 + H2O Plasmodium falciparum PSM2 auto-cleavage after F112 is complete after 20 min in 10 mM citrate pH 4.5 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification PSM2 is less active after maturation. At pH 2.2 PSM2 activity is observed only without maturation Plasmodium falciparum

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O bovine and human hemoglobin are used Plasmodium falciparum ?
-
 ?
Myoglobin + H2O horse myoglobin is used Plasmodium falciparum ?
-
 ?
p47phox-PX + H2O PX domain of p47phox, a cytosolic factor of the neutrophil NADPH oxydase is digested Plasmodium falciparum ?
-
 ?
plasmepsin 2 + H2O PSM2 auto-cleavage after F112 is complete after 20 min in 10 mM citrate pH 4.5 Plasmodium falciparum ?
-
 ?

Synonyms

Synonyms Comment Organism
plasmepsin 2
-
 Plasmodium falciparum
PSM2
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 assay at room temperature. At pH 4.5 the number of cleavage sites is lower at 0°C than at room temperature Plasmodium falciparum
-
-
 assay at, at pH 4.5 the number of cleavage sites is lower at 0°C than at room temperature Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2
-
 assay at, PSM2 produces more cleavage sites at pH 4.5 than at pH 2.2. At pH 2.2 PSM2 activity is observed only without maturation Plasmodium falciparum
4.5
-
 assay at, at pH 4.5 the number of cleavage sites is lower at 0°C than at room temperature. PSM2 produces more cleavage sites at pH 4.5 than at pH 2.2 Plasmodium falciparum