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Literature summary for 3.4.23.35 extracted from

  • MacKay, V.L.; Armstrong, J.; Yip, C.; Welch, S.; Walker, K.; Osborn, S.; Sheppard, P.; Forstrom, J.
    Characterization of the bar proteinase, an extracellular enzyme from the yeast Saccharomyces cerevisiae (1991), Struct. Funct. Asp. Proteinases (Dunn, Ben M. ; ed. ) Plenum Press, New York, , 161-172.
    View publication on PubMed
Search for more literature for 3.4.23.35

General Stability

General Stability Organism
Mutant enzyme undergoes extensive proteolysis during purification with little or no loss of activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information alpha factor derived peptides Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln, Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln, Trp-Leu-Gln-Leu-Lys-Pro-Gly; the reduced and alkylated B-chain of platelet-derived growth factor, synthetic peptides Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu, Ala-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu, Arg-Phe-Leu-Glu-(4-nitro)Phe-Lys-Pro-Gly-Gln-Pro, Lys-Pro-Glu-Ile-(4-nitro)Phe-Lys-Ser-Glu-Lys-Ile; Trp-Leu-Gln-Leu-Lys-Pro Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no Ca2+ or other divalent cation required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha Factor + H2O Saccharomyces cerevisiae i.e. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, involved in physiological changes preceding Saccharomyces cerevisiae cell fusion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 heterothallic strains
-
Saccharomyces cerevisiae
-
 baker's yeast mutant strains
-
Saccharomyces cerevisiae
-
 baker's yeast, wild-type
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein substantially modified post-translationally by N-glycosylation and probably also by O-glycosylation Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
purification from wild-type cells has been hampered because of the heterogeneous hyperglycosylation of the protein Saccharomyces cerevisiae
to near homogeneity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha Factor + H2O cleavage site: Leu6-Lys7 Saccharomyces cerevisiae Trp-His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr
-
 ?
alpha Factor + H2O strict substrate specificity Saccharomyces cerevisiae Trp-His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr
-
 ?
alpha Factor + H2O i.e. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, a Saccharomyces cerevisiae peptide mating pheromone secreted by mating-type alpha cells Saccharomyces cerevisiae Trp-His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr
-
 ?
alpha Factor + H2O i.e. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, involved in physiological changes preceding Saccharomyces cerevisiae cell fusion Saccharomyces cerevisiae ?
-
 ?
His-Trp-Leu-Gln-Leu-Arg-Pro-Gly-Gln-Pro-Met-Tyr + H2O cleavage site: Leu-Arg Saccharomyces cerevisiae His-Trp-Leu-Gln-Leu + Arg-Pro-Gly-Gln-Pro-Met-Tyr
-
 ?
His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr + H2O cleavage site: Leu-Lys Saccharomyces cerevisiae His-Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr
-
 ?
additional information substrate specificity Saccharomyces cerevisiae ?
-
 ?
additional information requires at least 4 amino acid residues on N-terminal and more than 4 on C-terminal side Saccharomyces cerevisiae ?
-
 ?
additional information no hydrolysis of alpha factor derived peptides Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr, His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln, Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln, Trp-Leu-Gln-Leu-Lys-Pro-Gly Saccharomyces cerevisiae ?
-
 ?
additional information Trp-Leu-Gln-Leu-Lys-Pro Saccharomyces cerevisiae ?
-
 ?
additional information the reduced and alkylated B-chain of platelet-derived growth factor, synthetic peptides Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu, Ala-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu, Arg-Phe-Leu-Glu-(4-nitro)Phe-Lys-Pro-Gly-Gln-Pro, Lys-Pro-Glu-Ile-(4-nitro)Phe-Lys-Ser-Glu-Lys-Ile Saccharomyces cerevisiae ?
-
 ?
Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr + H2O cleavage site: Leu-Lys Saccharomyces cerevisiae Trp-Leu-Gln-Leu + Lys-Pro-Gly-Gln-Pro-Met-Tyr
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.3
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
2.6 6.8 more than half-maximal activity at pH 2.6 and 6.8, slight activity at pH 1.1 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Saccharomyces cerevisiae