BRENDA - Enzyme Database show
show all sequences of 3.4.23.32

Studies on the catalytic mechanism of a glutamic peptidase

Kondo, M.Y.; Okamoto, D.N.; Santos, J.A.; Juliano, M.A.; Oda, K.; Pillai, B.; James, M.N.; Juliano, L.; Gouvea, I.E.; J. Biol. Chem. 285, 21437-21445 (2010)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetyl-Phe--Lys-Phe-(3S,4S)-phenylstatinyl-Leu-Arg-NH2
SGP transition state mimic inhibitor
Scytalidium lignicola
NaCl
a substantial decrease of the SGP catalytic efficiency (kcat/Km) is observed as the NaCl concentration is increases up to 1 M, while in the range up to 200 mM NaCl the effect is mainly in Km because there is no significant decrease in kcat values. A significant decrease in the kcat values is observed as the salt concentration is further increased from 200 to 800 mM NaCl
Scytalidium lignicola
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0002
-
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.00025
-
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.00026
-
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0003
-
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0004
-
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0004
-
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0004
-
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0005
-
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.00055
-
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0009
-
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0011
-
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0012
-
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0014
-
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0016
-
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0017
-
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.002
-
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0022
-
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0023
-
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.003
-
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0039
-
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.004
-
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0046
-
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0046
-
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Scytalidium lignicola
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLA + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLA + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLD + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLD + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLEPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLE + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLES + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLE + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLF + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLF + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLG + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLG + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLH + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLH + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLK + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLK + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLLPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLL + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLL + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLMPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLM + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLM + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLN + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLN + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLPPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLP + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLP + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLQ + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLQ + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLR + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLR + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLS + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLS + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLVPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLV + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLV + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLY + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLY + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
additional information
the enzyme shows preference for Phe and His over Leu, Ile, Val, Arg, and Lys. Peptides with positively charged amino acids Arg and Lys or aliphatic amino acids Leu, Ile, and Val are all poor substrates, 2-aminobenzoyl-KLIPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLISSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], and 2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] are resistant to hydrolysis
709118
Scytalidium lignicola
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.03
-
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.048
-
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.063
-
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.2
-
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.4
-
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.4
-
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.4
-
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.6
-
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.8
-
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
1.1
-
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
1.6
-
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3.5
-
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3.6
-
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
6
-
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
6.5
-
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
7.7
-
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
7.7
-
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
8
-
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
8.7
-
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
13
-
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
15
-
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
17
-
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
33
-
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
86
-
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
105
-
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
145
-
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
198
-
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
200
-
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Scytalidium lignicola
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
9.5
in the pH range 8.0-9.5, the enzyme becomes irreversibly inactivated
Scytalidium lignicola
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetyl-Phe--Lys-Phe-(3S,4S)-phenylstatinyl-Leu-Arg-NH2
SGP transition state mimic inhibitor
Scytalidium lignicola
NaCl
a substantial decrease of the SGP catalytic efficiency (kcat/Km) is observed as the NaCl concentration is increases up to 1 M, while in the range up to 200 mM NaCl the effect is mainly in Km because there is no significant decrease in kcat values. A significant decrease in the kcat values is observed as the salt concentration is further increased from 200 to 800 mM NaCl
Scytalidium lignicola
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0002
-
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.00025
-
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.00026
-
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0003
-
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0004
-
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0004
-
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0004
-
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0005
-
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.00055
-
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0008
-
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0009
-
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0011
-
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0012
-
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0014
-
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0016
-
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0017
-
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.002
-
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0022
-
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0023
-
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.003
-
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0039
-
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.004
-
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0046
-
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.0046
-
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLA + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLA + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLD + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLD + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLEPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLE + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLES + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLE + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLF + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLF + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLG + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLG + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLH + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLH + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLK + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLK + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLLPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLL + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLL + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLMPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLM + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLM + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLN + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLN + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLPPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLP + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLP + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLQ + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLQ + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLR + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLR + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLS + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLS + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLVPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLV + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLV + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLY + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
-
709118
Scytalidium lignicola
2-aminobenzoyl-KLY + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
-
?
additional information
the enzyme shows preference for Phe and His over Leu, Ile, Val, Arg, and Lys. Peptides with positively charged amino acids Arg and Lys or aliphatic amino acids Leu, Ile, and Val are all poor substrates, 2-aminobenzoyl-KLIPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLISSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], 2-aminobenzoyl-KLWSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine], and 2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] are resistant to hydrolysis
709118
Scytalidium lignicola
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.03
-
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.048
-
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.063
-
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.2
-
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.4
-
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.4
-
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.4
-
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.6
-
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
0.8
-
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
1.1
-
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
1.6
-
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3.5
-
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3.6
-
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
6
-
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
6.5
-
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
7.7
-
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
7.7
-
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
8
-
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
8.7
-
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
13
-
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
15
-
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
17
-
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
33
-
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
86
-
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
105
-
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
145
-
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
198
-
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
200
-
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Scytalidium lignicola
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
9.5
in the pH range 8.0-9.5, the enzyme becomes irreversibly inactivated
Scytalidium lignicola
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
20
-
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
30
-
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
100
-
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
150
-
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
160
-
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
200
-
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
250
-
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
400
-
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
700
-
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
800
-
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
1000
-
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
2600
-
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3700
-
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3700
-
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
4800
-
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
6400
-
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
16000
-
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
19000
-
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
19000
-
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
26000
-
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
35000
-
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
65000
-
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
66000
-
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
78000
-
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
131000
-
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
167000
-
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
330000
-
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
363000
-
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
20
-
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
30
-
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
100
-
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
150
-
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
160
-
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
200
-
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
250
-
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
400
-
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
700
-
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
800
-
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
1000
-
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
2600
-
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3700
-
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
3700
-
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
4800
-
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
6400
-
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
16000
-
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
19000
-
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
19000
-
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
26000
-
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
35000
-
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
65000
-
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
66000
-
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
78000
-
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
131000
-
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
167000
-
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
330000
-
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
363000
-
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
Scytalidium lignicola
Other publictions for EC 3.4.23.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709118
Kondo
Studies on the catalytic mecha ...
Scytalidium lignicola
J. Biol. Chem.
285
21437-21445
2010
-
-
-
-
-
-
2
28
-
-
-
-
-
2
-
-
-
-
-
-
-
-
34
-
-
-
-
28
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
28
-
-
-
-
-
-
-
-
-
-
-
-
34
-
-
-
-
28
1
-
1
-
-
-
-
-
28
28
688338
Pillai
Crystal structure of Scytalido ...
Scytalidium lignicola
J. Mol. Biol.
365
343-361
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685437
James
The peptidases from fungi and ...
Scytalidium lignicola
Biol. Chem.
387
1023-1029
2006
-
-
-
1
3
-
1
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
668804
Oda
-
Scytalidopepsin B ...
Scytalidium lignicola
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
219-221
2004
-
-
1
-
-
-
3
-
1
-
1
1
-
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
-
1
-
1
1
-
-
-
-
-
-
1
-
5
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
670664
Fujinaga
The molecular structure and ca ...
Scytalidium lignicola
Proc. Natl. Acad. Sci. USA
101
3364-3369
2004
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30559
Majima
-
Comparative study on the speci ...
Scytalidium lignicola
Agric. Biol. Chem.
52
787-793
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30762
Oda
-
Studies on new acid proteases ...
Scytalidium lignicola
Agric. Biol. Chem.
50
1995-2001
1986
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30765
Tsuru
Isolation and amino acid seque ...
Scytalidium lignicola
J. Biochem.
99
1537-1539
1986
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30764
Maita
Complete amino acid sequence o ...
Scytalidium lignicola
J. Biochem.
95
465-475
1984
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30767
Terashita
-
Streptomyces pepsin inhibitor- ...
Ganoderma lucidum
Agric. Biol. Chem.
48
1029-1035
1984
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
3
1
1
-
3
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
-
1
1
-
3
1
1
-
3
-
2
1
2
-
-
-
-
-
-
-
30758
Ichishima
-
Enzymatic cleavage of the hist ...
Scytalidium lignicola
Agric. Biol. Chem.
45
2391-2393
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30766
Terashita
-
Streptomyces pepsin inhibitor- ...
Lentinula edodes
Agric. Biol. Chem.
45
1937-1943
1981
-
-
-
-
-
-
2
-
-
-
2
-
-
1
-
1
1
-
-
1
1
-
2
1
1
-
3
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
1
1
-
1
1
-
2
1
1
-
3
-
2
-
2
-
-
-
-
-
-
-
30759
Morihara
Pepstatin-insenstive acid prot ...
Scytalidium lignicola
J. Biochem.
85
661-668
1979
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30755
Oda
-
Studies on new acid proteases ...
Scytalidium lignicola
Agric. Biol. Chem.
40
1221-1225
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30754
Oda
-
New acid proteases from Scytal ...
Scytalidium lignicola
Agric. Biol. Chem.
38
2435-2444
1974
-
-
-
-
-
-
3
-
-
1
1
-
-
1
-
1
1
-
-
1
1
-
2
-
1
1
3
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
1
-
-
-
1
1
-
1
1
-
2
-
1
1
3
-
1
1
2
-
-
-
-
-
-
-