BRENDA - Enzyme Database show
show all sequences of 3.4.23.32

New acid proteases from Scytalidium lignicolum M-133. II. Purification and some enzymatic properties of acid protease A and B of Scytalidium lignicolum ATCC 24568.

Oda, K.; Murao, S.; Agric. Biol. Chem. 38, 2435-2444 (1974)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Scytalidium lignicola
additional information
diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate; EDTA; methyl 2-diazoacetamidohexanoate; Streptomyces-pepsin inhibitor (S-PI); sulfhydryl reagents
Scytalidium lignicola
N-bromosuccinimide
-
Scytalidium lignicola
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CuCl2
10 mM, activates
Scytalidium lignicola
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
Scytalidium lignicolum, gel filtration
Scytalidium lignicola
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Scytalidium lignicola
-
ATCC 24568
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
no glycoprotein
Scytalidium lignicola
Purification (Commentary)
Commentary
Organism
-
Scytalidium lignicola
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Scytalidium lignicola
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Scytalidium lignicola
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
casein + H2O
-
30754
Scytalidium lignicola
hydrolyzed casein
-
-
-
-
casein + H2O
-
30754
Scytalidium lignicola A-2
hydrolyzed casein
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Scytalidium lignicola
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
68
37°C: about 30% of activity maximum, 68°C: about 50% of activity maximum
Scytalidium lignicola
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 1.5-5.5, 20 h, stable
Scytalidium lignicola
60
-
pH 4.0, 15 min, stable below
Scytalidium lignicola
68
-
pH 4.0, 15 min, complete loss of activity
Scytalidium lignicola
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2
2.2
-
Scytalidium lignicola
pH Range
pH Minimum
pH Maximum
Commentary
Organism
1.3
3.5
1.3: about 80% of activity maximum, 3.5: about 35% of activity maximum
Scytalidium lignicola
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
1.5
5
37°C, 20 h
Scytalidium lignicola
4
-
15 min, complete loss of activity at 68°C; stable below 60°C, 15 min
Scytalidium lignicola
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Scytalidium lignicola
additional information
diazoacetyl-DL-norleucine methyl ester; diisopropyl fluorophosphate; EDTA; methyl 2-diazoacetamidohexanoate; Streptomyces-pepsin inhibitor (S-PI); sulfhydryl reagents
Scytalidium lignicola
N-bromosuccinimide
-
Scytalidium lignicola
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CuCl2
10 mM, activates
Scytalidium lignicola
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
Scytalidium lignicolum, gel filtration
Scytalidium lignicola
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
no glycoprotein
Scytalidium lignicola
Purification (Commentary) (protein specific)
Commentary
Organism
-
Scytalidium lignicola
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Scytalidium lignicola
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Scytalidium lignicola
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
casein + H2O
-
30754
Scytalidium lignicola
hydrolyzed casein
-
-
-
-
casein + H2O
-
30754
Scytalidium lignicola A-2
hydrolyzed casein
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Scytalidium lignicola
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
37
68
37°C: about 30% of activity maximum, 68°C: about 50% of activity maximum
Scytalidium lignicola
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 1.5-5.5, 20 h, stable
Scytalidium lignicola
60
-
pH 4.0, 15 min, stable below
Scytalidium lignicola
68
-
pH 4.0, 15 min, complete loss of activity
Scytalidium lignicola
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2
2.2
-
Scytalidium lignicola
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
1.3
3.5
1.3: about 80% of activity maximum, 3.5: about 35% of activity maximum
Scytalidium lignicola
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
1.5
5
37°C, 20 h
Scytalidium lignicola
4
-
15 min, complete loss of activity at 68°C; stable below 60°C, 15 min
Scytalidium lignicola
Other publictions for EC 3.4.23.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709118
Kondo
Studies on the catalytic mecha ...
Scytalidium lignicola
J. Biol. Chem.
285
21437-21445
2010
-
-
-
-
-
-
2
28
-
-
-
-
-
2
-
-
-
-
-
-
-
-
34
-
-
-
-
28
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
28
-
-
-
-
-
-
-
-
-
-
-
-
34
-
-
-
-
28
1
-
1
-
-
-
-
-
28
28
688338
Pillai
Crystal structure of Scytalido ...
Scytalidium lignicola
J. Mol. Biol.
365
343-361
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
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1
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-
685437
James
The peptidases from fungi and ...
Scytalidium lignicola
Biol. Chem.
387
1023-1029
2006
-
-
-
1
3
-
1
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
1
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1
3
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1
-
1
-
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-
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-
-
-
-
1
-
-
-
-
1
-
-
-
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-
-
-
-
-
668804
Oda
-
Scytalidopepsin B ...
Scytalidium lignicola
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
219-221
2004
-
-
1
-
-
-
3
-
1
-
1
1
-
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
-
1
-
1
1
-
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-
-
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-
1
-
5
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
670664
Fujinaga
The molecular structure and ca ...
Scytalidium lignicola
Proc. Natl. Acad. Sci. USA
101
3364-3369
2004
-
-
-
1
-
-
-
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-
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-
2
-
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-
1
-
-
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-
1
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-
1
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-
1
-
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-
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-
-
-
-
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-
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-
30559
Majima
-
Comparative study on the speci ...
Scytalidium lignicola
Agric. Biol. Chem.
52
787-793
1988
-
-
-
-
-
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1
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1
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-
1
-
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-
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-
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-
-
-
-
30762
Oda
-
Studies on new acid proteases ...
Scytalidium lignicola
Agric. Biol. Chem.
50
1995-2001
1986
-
-
-
-
-
-
2
-
-
-
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-
1
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2
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2
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-
2
-
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30765
Tsuru
Isolation and amino acid seque ...
Scytalidium lignicola
J. Biochem.
99
1537-1539
1986
-
-
-
-
-
-
2
-
-
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-
1
-
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2
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-
30764
Maita
Complete amino acid sequence o ...
Scytalidium lignicola
J. Biochem.
95
465-475
1984
-
-
-
-
-
-
-
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-
1
1
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-
1
-
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1
1
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-
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-
-
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-
-
-
-
-
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-
30767
Terashita
-
Streptomyces pepsin inhibitor- ...
Ganoderma lucidum
Agric. Biol. Chem.
48
1029-1035
1984
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
3
1
1
-
3
-
2
1
2
-
-
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-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
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1
-
1
1
-
3
1
1
-
3
-
2
1
2
-
-
-
-
-
-
-
30758
Ichishima
-
Enzymatic cleavage of the hist ...
Scytalidium lignicola
Agric. Biol. Chem.
45
2391-2393
1981
-
-
-
-
-
-
-
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1
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1
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1
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30766
Terashita
-
Streptomyces pepsin inhibitor- ...
Lentinula edodes
Agric. Biol. Chem.
45
1937-1943
1981
-
-
-
-
-
-
2
-
-
-
2
-
-
1
-
1
1
-
-
1
1
-
2
1
1
-
3
-
2
-
2
-
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2
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2
-
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1
1
-
1
1
-
2
1
1
-
3
-
2
-
2
-
-
-
-
-
-
-
30759
Morihara
Pepstatin-insenstive acid prot ...
Scytalidium lignicola
J. Biochem.
85
661-668
1979
-
-
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1
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1
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5
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1
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5
-
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-
-
-
-
-
-
-
-
-
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-
-
-
30755
Oda
-
Studies on new acid proteases ...
Scytalidium lignicola
Agric. Biol. Chem.
40
1221-1225
1976
-
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1
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1
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1
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30754
Oda
-
New acid proteases from Scytal ...
Scytalidium lignicola
Agric. Biol. Chem.
38
2435-2444
1974
-
-
-
-
-
-
3
-
-
1
1
-
-
1
-
1
1
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1
1
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2
-
1
1
3
-
1
1
2
-
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3
-
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1
1
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1
1
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1
1
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2
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1
1
3
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1
1
2
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