BRENDA - Enzyme Database show
show all sequences of 3.4.23.31

Purification and characterization of acid proteinase C of Scytalidium lignicolum ATCC 24568

Oda, K.; Torishima, H.; Murao, S.; Agric. Biol. Chem. 50, 651-658 (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-Microbial alkaline proteinase inhibitor
weak
Scytalidium lignicola
additional information
a related enzyme from the same organism, proteinase C is also insensitive to these inhibitors; diazoacetyl-DL-norleucine methyl ester; methyl 2-diazoacetamidohexanoate, 1,2-epoxy-3-(4-nitrophenyl)propane; Streptomyces-pepsin inhibitor; talopeptin (MK-1, metal proteinase inhibitor); thiolstatin (thiol proteinase inhibitor)
Scytalidium lignicola
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester
enzyme form C
Scytalidium lignicola
1
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester
enzyme form A-2
Scytalidium lignicola
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
Scytalidium lignicolum, gel filtration
Scytalidium lignicola
40600
-
Scytalidium lignicolum, sedimentation equilibrium analysis
Scytalidium lignicola
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Scytalidium lignicola
-
A-2; ATCC 24568; C
-
Scytalidium lignicola A-2
-
A-2
-
Scytalidium lignicola C
-
C
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
30% (expressed as the glucose equivalent)
Scytalidium lignicola
Purification (Commentary)
Commentary
Organism
proteinase C
Scytalidium lignicola
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Scytalidium lignicola
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Scytalidium lignicola
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola C
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola A-2
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola C
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola A-2
?
-
-
-
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O
at high rate
30756
Scytalidium lignicola
?
-
-
-
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O
at high rate
30756
Scytalidium lignicola C
?
-
-
-
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O
at high rate
30756
Scytalidium lignicola A-2
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Scytalidium lignicola
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 3-5, 20 h, stable
Scytalidium lignicola
55
-
pH 4.8, 15 min, stable below
Scytalidium lignicola
68
-
pH 4.8, 15 min, complete loss of activity
Scytalidium lignicola
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2
-
casein
Scytalidium lignicola
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3
5
37°C, 20 h, stable
Scytalidium lignicola
4.8
-
15 min, stable below 55°C, complete loss of activity at 68°C
Scytalidium lignicola
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-Microbial alkaline proteinase inhibitor
weak
Scytalidium lignicola
additional information
a related enzyme from the same organism, proteinase C is also insensitive to these inhibitors; diazoacetyl-DL-norleucine methyl ester; methyl 2-diazoacetamidohexanoate, 1,2-epoxy-3-(4-nitrophenyl)propane; Streptomyces-pepsin inhibitor; talopeptin (MK-1, metal proteinase inhibitor); thiolstatin (thiol proteinase inhibitor)
Scytalidium lignicola
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester
enzyme form C
Scytalidium lignicola
1
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester
enzyme form A-2
Scytalidium lignicola
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
Scytalidium lignicolum, gel filtration
Scytalidium lignicola
40600
-
Scytalidium lignicolum, sedimentation equilibrium analysis
Scytalidium lignicola
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
30% (expressed as the glucose equivalent)
Scytalidium lignicola
Purification (Commentary) (protein specific)
Commentary
Organism
proteinase C
Scytalidium lignicola
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Scytalidium lignicola
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Scytalidium lignicola
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola C
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola A-2
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola C
?
-
-
-
-
N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
30756
Scytalidium lignicola A-2
?
-
-
-
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O
at high rate
30756
Scytalidium lignicola
?
-
-
-
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O
at high rate
30756
Scytalidium lignicola C
?
-
-
-
-
Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O
at high rate
30756
Scytalidium lignicola A-2
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Scytalidium lignicola
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
pH 3-5, 20 h, stable
Scytalidium lignicola
55
-
pH 4.8, 15 min, stable below
Scytalidium lignicola
68
-
pH 4.8, 15 min, complete loss of activity
Scytalidium lignicola
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2
-
casein
Scytalidium lignicola
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3
5
37°C, 20 h, stable
Scytalidium lignicola
4.8
-
15 min, stable below 55°C, complete loss of activity at 68°C
Scytalidium lignicola
Other publictions for EC 3.4.23.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668806
Murao
-
Scytalidopepsin A ...
Scytalidium lignicola
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
224-225
2004
-
-
-
-
-
-
1
-
1
-
1
1
-
1
-
1
-
-
-
-
1
-
5
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
1
-
-
1
-
-
-
1
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
30559
Majima
-
Comparative study on the speci ...
Scytalidium lignicola
Agric. Biol. Chem.
52
787-793
1988
-
-
-
-
-
-
1
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-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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1
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-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
30756
Oda
-
Purification and characterizat ...
Scytalidium lignicola, Scytalidium lignicola A-2, Scytalidium lignicola C
Agric. Biol. Chem.
50
651-658
1986
-
-
-
-
-
-
2
2
-
-
2
-
-
8
-
1
1
-
-
1
1
-
9
-
1
-
3
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
2
-
-
-
1
1
-
1
1
-
9
-
1
-
3
-
1
-
2
-
-
-
-
-
-
-
30757
Oda
-
Kinetic studies on S-PI(Pepsta ...
Scytalidium lignicola, Scytalidium lignicola A-2
Agric. Biol. Chem.
50
659-663
1986
-
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
4
-
-
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-
-
-
-
-
-
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-
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-
-
-
-
-
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-
-
1
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-
-
-
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-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30762
Oda
-
Studies on new acid proteases ...
Scytalidium lignicola, Scytalidium lignicola A-2
Agric. Biol. Chem.
50
1995-2001
1986
-
-
-
-
-
-
-
-
-
-
-
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-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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1
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-
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-
-
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-
-
-
-
-
-
-
-
30758
Ichishima
-
Enzymatic cleavage of the hist ...
Scytalidium lignicola
Agric. Biol. Chem.
45
2391-2393
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
2
-
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-
-
-
-
-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30759
Morihara
Pepstatin-insenstive acid prot ...
Scytalidium lignicola, Scytalidium lignicola A-2
J. Biochem.
85
661-668
1979
-
-
-
-
-
-
7
12
-
-
-
-
-
7
-
-
-
-
-
-
-
-
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
12
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
30763
Murao
-
Studies on new acid proteases ...
Scytalidium lignicola
Agric. Biol. Chem.
42
1343-1349
1978
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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1
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-
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-
-
-
-
-
-
-
-
-
30755
Oda
-
Studies on new acid proteases ...
Scytalidium lignicola
Agric. Biol. Chem.
40
1221-1225
1976
-
-
-
-
-
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1
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1
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1
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-
30760
Oda
-
Pepstatin-insensitive acid pro ...
Scytalidium lignicola, Scytalidium lignicola A-2
Agric. Biol. Chem.
40
859-866
1976
-
-
-
-
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-
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1
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7
-
1
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27
-
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1
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1
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27
-
-
-
-
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-
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-
-
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-
-
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-
30754
Oda
-
New acid proteases from Scytal ...
Scytalidium lignicola, Scytalidium lignicola A-2
Agric. Biol. Chem.
38
2435-2444
1974
-
-
-
-
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4
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2
1
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7
-
1
1
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-
1
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2
-
1
1
3
-
1
1
2
-
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4
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2
1
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1
1
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1
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2
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1
1
3
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1
1
2
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