Literature summary for 3.4.23.29 extracted from

Fujimoto, Z.; Fujii, Y.; Kaneko, S.; Kobayashi, H.; Mizuno, H.
Crystal structure of aspartic proteinase from Irpex lacteus in complex with inhibitor pepstatin (2004), J. Mol. Biol., 341, 1227-1235.

Search for more literature for 3.4.23.29

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in complex with inhibitor pepstatin, hanging drop vapour diffusion method, 0.005 ml of 10 mg/ml protein is mixed with 0.005 ml of reservoir solution containing 50% ammonium sulfate in 10 mM sodium citrate-sulfate buffer, pH 5.4, 20°C, X-ray diffraction structure determination and analysis at 1.3 A resolution, structure modeling	Irpex lacteus

Inhibitors

Inhibitors	Comment	Organism	Structure
pepstatin	binding structure	Irpex lacteus

Organism

Organism	UniProt	Comment	Textmining
Irpex lacteus	-	formerly Irpex lacteus	-

Reaction

Reaction	Comment	Organism	Reaction ID
Milk clotting activity, broad specificity, but fails to cleave Leu15-Tyr or Tyr16-Leu of insulin B chain	catalytic mechanism, transition state model, substrate binding structure and mechanism	Irpex lacteus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme requires hydrophobic amino acids at P1 and/or P1' positions and at P3 and/or P4 positions, the enzyme shows high milk-clotting activity	Irpex lacteus	?	-	?

Synonyms

Synonyms	Comment	Organism
ILAP	-	Irpex lacteus
Irpex lacteus aspartic protease	-	Irpex lacteus
milk-clotting enzyme	-	Irpex lacteus

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Release 2023.1 (January 2023)