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Literature summary for 3.4.23.29 extracted from

  • Kobayashi, H.; Kusakabe, I.; Murakami, K.
    Purification and characterization of a pepstatin-insensitive carboxyl proteinase from Polyporus tulipiferae (Irpex lacteus) (1985), Agric. Biol. Chem., 49, 2393-2397.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,2-epoxy-3-(4-nitrophenoxy)propane not Irpex lacteus
chymostatin
-
Irpex lacteus
Diazoacetyl-D,L-norleucine methyl ester not Irpex lacteus
Hg2+
-
Irpex lacteus
N-p-toluene sulfonyl-L-Phe chloromethyl ketone
-
Irpex lacteus
pepstatin not Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
formerly Irpex lacteus
-

Purification (Commentary)

Purification (Comment) Organism
-
Irpex lacteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Phe-Gly-Ala + H2O
-
Irpex lacteus Ala-Phe + Gly-Ala
-
?
Ala-Phe-Leu-Ala + H2O
-
Irpex lacteus Ala-Phe + Leu-Ala
-
?
Gly-Phe-Leu-Ala + H2O
-
Irpex lacteus Gly-Phe + Leu-Ala
-
?
Hemoglobin + H2O
-
Irpex lacteus Proteolytically cleaved hemogobin
-
?
additional information milk clotting activity Irpex lacteus ?
-
?
Phe-Ala-Ala-Ala + H2O
-
Irpex lacteus Phe-Ala + Ala-Ala
-
?
Phe-Glu-Ala-Ala + H2O
-
Irpex lacteus Phe-Glu + Ala-Ala
-
?
Phe-Gly-Ala-Ala + H2O
-
Irpex lacteus Phe-Gly + Ala-Ala
-
?
Phe-Leu-Ala-Ala + H2O
-
Irpex lacteus Phe-Leu + Ala-Ala
-
?
Phe-Lys-Ala-Ala + H2O
-
Irpex lacteus Phe-Lys + Ala-Ala
-
?
Phe-Tyr-Ala-Ala + H2O
-
Irpex lacteus Phe-Tyr + Ala-Ala
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 4.6, 15 min, stable Irpex lacteus
45
-
pH 4.6, 15 min, complete inactivation Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8
-
hemoglobin Irpex lacteus
4
-
Phe-Leu-Ala-Ala Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.6
-
15 min, complete inactivation at 45°C, stable at 30°C Irpex lacteus