BRENDA - Enzyme Database show
show all sequences of 3.4.23.29

Purification and characterization of a pepstatin-insensitive carboxyl proteinase from Polyporus tulipiferae (Irpex lacteus)

Kobayashi, H.; Kusakabe, I.; Murakami, K.; Agric. Biol. Chem. 49, 2393-2397 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
not
Irpex lacteus
chymostatin
-
Irpex lacteus
Diazoacetyl-D,L-norleucine methyl ester
not
Irpex lacteus
Hg2+
-
Irpex lacteus
N-p-toluene sulfonyl-L-Phe chloromethyl ketone
-
Irpex lacteus
pepstatin
not
Irpex lacteus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Irpex lacteus
-
formerly Irpex lacteus
-
Purification (Commentary)
Commentary
Organism
-
Irpex lacteus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Irpex lacteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ala-Phe-Gly-Ala + H2O
-
30730
Irpex lacteus
Ala-Phe + Gly-Ala
-
30730
Irpex lacteus
-
Ala-Phe-Leu-Ala + H2O
-
30730
Irpex lacteus
Ala-Phe + Leu-Ala
-
30730
Irpex lacteus
-
Gly-Phe-Leu-Ala + H2O
-
30730
Irpex lacteus
Gly-Phe + Leu-Ala
-
30730
Irpex lacteus
-
Hemoglobin + H2O
-
30730
Irpex lacteus
Proteolytically cleaved hemogobin
-
-
-
-
additional information
milk clotting activity
30730
Irpex lacteus
?
-
-
-
-
Phe-Ala-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Ala + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Glu-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Glu + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Gly-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Gly + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Leu-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Leu + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Lys-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Lys + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Tyr-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Tyr + Ala-Ala
-
30730
Irpex lacteus
-
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
pH 4.6, 15 min, stable
Irpex lacteus
45
-
pH 4.6, 15 min, complete inactivation
Irpex lacteus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8
-
hemoglobin
Irpex lacteus
4
-
Phe-Leu-Ala-Ala
Irpex lacteus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4.6
-
15 min, complete inactivation at 45C, stable at 30C
Irpex lacteus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
not
Irpex lacteus
chymostatin
-
Irpex lacteus
Diazoacetyl-D,L-norleucine methyl ester
not
Irpex lacteus
Hg2+
-
Irpex lacteus
N-p-toluene sulfonyl-L-Phe chloromethyl ketone
-
Irpex lacteus
pepstatin
not
Irpex lacteus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Irpex lacteus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Irpex lacteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ala-Phe-Gly-Ala + H2O
-
30730
Irpex lacteus
Ala-Phe + Gly-Ala
-
30730
Irpex lacteus
-
Ala-Phe-Leu-Ala + H2O
-
30730
Irpex lacteus
Ala-Phe + Leu-Ala
-
30730
Irpex lacteus
-
Gly-Phe-Leu-Ala + H2O
-
30730
Irpex lacteus
Gly-Phe + Leu-Ala
-
30730
Irpex lacteus
-
Hemoglobin + H2O
-
30730
Irpex lacteus
Proteolytically cleaved hemogobin
-
-
-
-
additional information
milk clotting activity
30730
Irpex lacteus
?
-
-
-
-
Phe-Ala-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Ala + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Glu-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Glu + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Gly-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Gly + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Leu-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Leu + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Lys-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Lys + Ala-Ala
-
30730
Irpex lacteus
-
Phe-Tyr-Ala-Ala + H2O
-
30730
Irpex lacteus
Phe-Tyr + Ala-Ala
-
30730
Irpex lacteus
-
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
pH 4.6, 15 min, stable
Irpex lacteus
45
-
pH 4.6, 15 min, complete inactivation
Irpex lacteus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.8
-
hemoglobin
Irpex lacteus
4
-
Phe-Leu-Ala-Ala
Irpex lacteus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4.6
-
15 min, complete inactivation at 45C, stable at 30C
Irpex lacteus
Other publictions for EC 3.4.23.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668788
Kobayashi
-
Polyporopepsin ...
Irpex lacteus
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
113-115
2004
-
-
1
1
-
-
2
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
6
1
-
-
1
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
2
-
-
-
-
1
-
-
-
1
1
-
-
-
-
6
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
669833
Fujimoto
Crystal structure of aspartic ...
Irpex lacteus
J. Mol. Biol.
341
1227-1235
2004
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30729
Kobayashi
-
Cloning and sequence analysis ...
Irpex lacteus
Agric. Biol. Chem.
53
1927-1933
1989
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30730
Kobayashi
-
Purification and characterizat ...
Irpex lacteus
Agric. Biol. Chem.
49
2393-2397
1985
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
11
-
-
-
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
11
-
-
-
2
-
2
-
1
-
-
-
-
-
-
-
30728
Kobayashi
-
Substrate specificity of a car ...
Irpex lacteus
Agric. Biol. Chem.
47
1921-1923
1983
-
-
-
-
-
-
3
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
30731
Kawai
-
Studies on the milk-clotting e ...
Irpex lacteus
Agric. Biol. Chem.
40
1463-1469
1976
-
-
-
1
-
-
2
-
-
-
3
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
3
-
-
-
-
1
-
-
-
-
1
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-