BRENDA - Enzyme Database show
show all sequences of 3.4.23.29

Substrate specificity of a carboxyl proteinase from Irpex lacteus

Kobayashi, H.; Kusakabe, I.; Murakami, K.; Agric. Biol. Chem. 47, 1921-1923 (1983)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Irpex lacteus
Diazoacetyl-D,L-norleucine methyl ester
-
Irpex lacteus
pepstatin
-
Irpex lacteus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
Polyporus tulipiferae
Irpex lacteus
39000
-
1 * 39000, Polyporus tulipiferae, SDS-PAGE
Irpex lacteus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Irpex lacteus
-
formerly Irpex lacteus
-
Purification (Commentary)
Commentary
Organism
-
Irpex lacteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Angiotensin I + H2O
hydrolyzes Tyr4-Ile5 bond much more rapidly than the Val3-Tyr4 bond
30728
Irpex lacteus
Proteolytically cleaved angiotensin
-
-
-
-
Hemoglobin + H2O
-
30728
Irpex lacteus
Proteolytically cleaved hemogobin
-
-
-
-
additional information
milk clotting activity
30728
Irpex lacteus
?
-
-
-
-
Oxidized B-chain of insulin + H2O
peptide bonds mainly susceptible to the enzyme: Leu11-Val12, Ala14-Leu15, Phe24-Phe25, Thr27-Pro28
30728
Irpex lacteus
Proteolytically cleaved insulin B-chain
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 39000, Polyporus tulipiferae, SDS-PAGE
Irpex lacteus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
hemoglobin
Irpex lacteus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Irpex lacteus
Diazoacetyl-D,L-norleucine methyl ester
-
Irpex lacteus
pepstatin
-
Irpex lacteus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
Polyporus tulipiferae
Irpex lacteus
39000
-
1 * 39000, Polyporus tulipiferae, SDS-PAGE
Irpex lacteus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Irpex lacteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Angiotensin I + H2O
hydrolyzes Tyr4-Ile5 bond much more rapidly than the Val3-Tyr4 bond
30728
Irpex lacteus
Proteolytically cleaved angiotensin
-
-
-
-
Hemoglobin + H2O
-
30728
Irpex lacteus
Proteolytically cleaved hemogobin
-
-
-
-
additional information
milk clotting activity
30728
Irpex lacteus
?
-
-
-
-
Oxidized B-chain of insulin + H2O
peptide bonds mainly susceptible to the enzyme: Leu11-Val12, Ala14-Leu15, Phe24-Phe25, Thr27-Pro28
30728
Irpex lacteus
Proteolytically cleaved insulin B-chain
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 39000, Polyporus tulipiferae, SDS-PAGE
Irpex lacteus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
hemoglobin
Irpex lacteus
Other publictions for EC 3.4.23.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668788
Kobayashi
-
Polyporopepsin ...
Irpex lacteus
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
113-115
2004
-
-
1
1
-
-
2
-
-
-
1
-
-
1
-
1
1
-
-
-
-
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6
1
-
-
1
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
2
-
-
-
-
1
-
-
-
1
1
-
-
-
-
6
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
669833
Fujimoto
Crystal structure of aspartic ...
Irpex lacteus
J. Mol. Biol.
341
1227-1235
2004
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-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30729
Kobayashi
-
Cloning and sequence analysis ...
Irpex lacteus
Agric. Biol. Chem.
53
1927-1933
1989
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30730
Kobayashi
-
Purification and characterizat ...
Irpex lacteus
Agric. Biol. Chem.
49
2393-2397
1985
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
11
-
-
-
2
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
11
-
-
-
2
-
2
-
1
-
-
-
-
-
-
-
30728
Kobayashi
-
Substrate specificity of a car ...
Irpex lacteus
Agric. Biol. Chem.
47
1921-1923
1983
-
-
-
-
-
-
3
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
30731
Kawai
-
Studies on the milk-clotting e ...
Irpex lacteus
Agric. Biol. Chem.
40
1463-1469
1976
-
-
-
1
-
-
2
-
-
-
3
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
1
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-
-
2
-
-
-
-
3
-
-
-
-
1
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-
-
1
-
-
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2
-
1
-
1
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-
-
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