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Literature summary for 3.4.23.25 extracted from
- Bakers yeast protease A purification and enzymatic and molecular properties (1982), Arch. Biochem. Biophys., 213, 426-433.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization inactivates | Saccharomyces cerevisiae |
| repeated freezing and thawing inactivates | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pepstatin | - |
Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
hemoglobin | - |
Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sugar content: 8.3% | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM imidazole buffer, pH 7.0, after 3 years, 40% of the original activity is retained | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| casein + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| Hemoglobin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | inactivates uridine nucleosidase | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
casein, azocasein | Saccharomyces cerevisiae |
| 3.2 | - |
hemoglobin | Saccharomyces cerevisiae |
| 4.7 | - |
inactivation of nucleosidase | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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