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Literature summary for 3.4.23.25 extracted from
- Characterization of proteinase A glycoforms from recombinant Saccharomyces cerevisiae (1993), Biotechnol. Appl. Biochem., 18, 377-388.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | overexpression results in secretion | Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40760 | - |
laser desorption mass spectroscopy, a second form with MW 38132 isolated from the culture medium is an underglycosylated form lacking the carbohydrate moiety at Asn269 | Saccharomyces cerevisiae |
| 42000 | - |
1 * 42000, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
carrying the gene PEP4 which encodes the prepro-proteinase A on multicopy plasmids, 7:3 mixture of two different forms of enzyme: 40000 MW and 42000 MW | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains carbohydrate moieties at Asn68 and Asn269 | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Saccharomyces cerevisiae | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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