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Literature summary for 3.4.23.23 extracted from
- Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris effect on secretion, activity and thermo-stability (2018), AMB Express, 8, 157 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Mucor circinelloides |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N331Q | the mutant enzyme is not glycosylated | Mucor circinelloides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mucor circinelloides | U5HT00 | - |
- |
| Mucor circinelloides DSM 2183 | U5HT00 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Mucor circinelloides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap column chromatography | Mucor circinelloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N-dimethyl-casein + H2O | - |
Mucor circinelloides | L-tyrosine + ? | - |
? |  |
| N,N-dimethyl-casein + H2O | - |
Mucor circinelloides DSM 2183 | L-tyrosine + ? | - |
? | |
| skimmed milk + H2O | - |
Mucor circinelloides | ? | - |
? | |
| skimmed milk + H2O | - |
Mucor circinelloides DSM 2183 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, deglycosylated protein, SDS-PAGE | Mucor circinelloides |
| ? | x * 37000, glycosylated protein, SDS-PAGE | Mucor circinelloides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aspartic protease | - |
Mucor circinelloides |
| MCAP | - |
Mucor circinelloides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Mucor circinelloides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | upon 60 min incubation period enzyme activity begins to decrease at a temperature above of 50 °C. After 1 h of incubation at 55°C, 75% of the unpurified enzyme's activity is retained | Mucor circinelloides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
- |
Mucor circinelloides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | 3.8 | the enzyme remains stable after 1 h at pH 3.3-3.6. The activity drops to about 60% and about 50% after 1 h at pH 3.7 and 3.8, respectively | Mucor circinelloides |
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Release 2023.1 (January 2023)
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