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Literature summary for 3.4.23.23 extracted from

  • da Silva, R.R.; Souto, T.B.; de Oliveira, T.B.; de Oliveira, L.C.; Karcher, D.; Juliano, M.A.; Juliano, L.; de Oliveira, A.H.; Rodrigues, A.; Rosa, J.C.; Cabral, H.
    Evaluation of the catalytic specificity, biochemical properties, and milk clotting abilities of an aspartic peptidase from Rhizomucor miehei (2016), J. Ind. Microbiol. Biotechnol., 43, 1059-1069 .
    View publication on PubMed
Search for more literature for 3.4.23.23

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 100 mM, 50% residual activity Rhizomucor miehei
pepstatin A 0.2 mM, 40% residual activity Rhizomucor miehei
SDS 0.02%, 50% residual activity Rhizomucor miehei
Triton X-100 0.2%, 60% residual activity Rhizomucor miehei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000036
-
 2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.000045
-
 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.00011
-
 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.00015
-
 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.0004
-
 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.0005
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.00085
-
 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 5 mM, 16% residual activity Rhizomucor miehei
Ca2+ 5 mM, 44% residual activity Rhizomucor miehei
Co2+ 5 mM, 36% residual activity Rhizomucor miehei
Cu2+ 5 mM, 0.3% residual activity Rhizomucor miehei
Mn2+ 5 mM, 36% residual activity Rhizomucor miehei

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1057
-
 pH 6.5, 45°C Rhizomucor miehei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + MSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + SWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O
-
 Rhizomucor miehei 2-aminobenzoyl-KLR + WSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine + H2O substrate derived from peptide sequence of kappa-casein Rhizomucor miehei 2-aminobenzoyl-LSF + MAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine
-
 ?
additional information enzyme shows a preference for aromatic, basic, and nonpolar amino acids, particularly methionine, with specific cleavage of the peptide bond between phenylalanine and methionine Rhizomucor miehei ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Rhizomucor miehei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Rhizomucor miehei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 90% of maximum activity Rhizomucor miehei
65
-
 80% of maximum activity Rhizomucor miehei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable a up to, for 60 min Rhizomucor miehei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.045
-
 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.12
-
 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.15
-
 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.35
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
0.5
-
 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
1.1
-
 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rhizomucor miehei

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 80% of maximum activity Rhizomucor miehei

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5
-
 Rhizomucor miehei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
700
-
 2-aminobenzoyl-KLRSSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
1000
-
 2-aminobenzoyl-KLRWSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
1000
-
 2-aminobenzoyl-KLRSSMQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
1091
-
 2-aminobenzoyl-KLRSWKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
1250
-
 2-aminobenzoyl-KLRMSKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
1294
-
 2-aminobenzoyl-KLRSKKQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei
4722
-
 2-aminobenzoyl-LSFMAIQ-N1-(2,4-dinitrophenyl)ethane-1,2-diamine pH 6.5, 45°C Rhizomucor miehei