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Literature summary for 3.4.23.23 extracted from
- Milk-clotting enzyme for Mucor pusillus var Lindt (1970), Methods Enzymol., 19, 446-459.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Rhizomucor pusillus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Iodine | complete inhibition at pH 6.7, little loss of activity at pH 3.0-5.0 | Rhizomucor pusillus |  |
| additional information | not: PCMB, iodoacetamide, NEM | Rhizomucor pusillus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizomucor pusillus |
| 29000 | 32500 | Mucor pusillus, gel filtration, sedimentation and diffusion data | Rhizomucor pusillus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor pusillus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| photooxidation, mediated by methylene blue, inactivates | Rhizomucor pusillus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizomucor pusillus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizomucor pusillus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl-Gly-Pro-L-Leu-Gly + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| Benzyloxycarbonyl-L-Glu-L-Tyr + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| Benzyloxycarbonyl-L-Phe-L-Leu + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| Benzyloxycarbonyl-L-Phe-L-Tyr + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| benzyloxycarbonyl-L-Tyr-L-Leu + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| Hemoglobin + H2O | - |
Rhizomucor pusillus | Hydrolyzed hemoglobin | - |
? | |
| Kappa-casein + H2O | - |
Rhizomucor pusillus | Hydrolyzed kappa-casein | - |
? | |
| L-Leu-Gly + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| L-Leu-Gly-Gly + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| additional information | milk clotting activity | Rhizomucor pusillus | ? | - |
? | |
| Peptides + H2O | - |
Rhizomucor pusillus | Hydrolyzed peptides | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizomucor pusillus |
| 38 | - |
pH 3.0-6.0, 8 days, more than 90% of activity retained | Rhizomucor pusillus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
hemoglobin | Rhizomucor pusillus |
| 4.5 | - |
casein | Rhizomucor pusillus |
| 5.5 | - |
milk coagulation | Rhizomucor pusillus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
60°C, 10 min, highest stability | Rhizomucor pusillus |
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Release 2023.1 (January 2023)
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