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Literature summary for 3.4.23.21 extracted from

  • Ichishima, E.; Ojima, M.; Yamagata, Y.; Hanzawa, S.; Nakamura, T.
    Molecular and enzymatic properties of an aspartic proteinase from Rhizopus hangchow (1995), Phytochemistry, 38, 27-30.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Diazoacetyl-DL-norleucine methyl ester
-
Rhizopus arrhizus
pepstatin A Rhizopus arrhizus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
Rhizopus hangchow, FPLC gel filtration Rhizopus arrhizus
37600
-
1 * 37600, Rhizopus hangchow, SDS-PAGE Rhizopus arrhizus

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhizopus arrhizus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rhizopus arrhizus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Rhizopus arrhizus ?
-
?
additional information milk clotting activity Rhizopus arrhizus ?
-
?
additional information trypsinogen activation Rhizopus arrhizus ?
-
?
Oxidized insulin B-chain + H2O primarily the Leu15-Tyr16 bond and the Tyr16-Leu17 bond is hydrolyzed, additional cleavage of the bonds Ala14-Leu15 and Phe24-Phe25 Rhizopus arrhizus Proteolytically cleaved oxidized insulin B-chain
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37600, Rhizopus hangchow, SDS-PAGE Rhizopus arrhizus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
pH 3, 5 min, stable Rhizopus arrhizus
50
-
pH 3, 5 min, complete inactivation Rhizopus arrhizus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4 trypsinogen activation Rhizopus arrhizus