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Literature summary for 3.4.23.20 extracted from
- Single step purification via magnetic nanoparticles of new broad pH active protease from Penicillium aurantiogriseum (2018), Protein Expr. Purif., 147, 22-28 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 10.55% inhibition at 10 mM | Penicillium aurantiogriseum |  |
| additional information | not inhibited by EDTA and iodoacetic acid | Penicillium aurantiogriseum | |
| pepstatin A | 55.08% inhibition at 10 mM | Penicillium aurantiogriseum | |
| phenylmethylsulfonyl fluoride | complete inhibition at 10 mM | Penicillium aurantiogriseum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium aurantiogriseum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using magnetite nanoparticles with azocasein immobilized | Penicillium aurantiogriseum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Penicillium aurantiogriseum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Penicillium aurantiogriseum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Penicillium aurantiogriseum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 55 | the purified enzyme keeps more than 48% activity from 15 to 55°C | Penicillium aurantiogriseum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | the purified enzyme does not lose any activity over 48 h at 25°C. The protease shows an activity loss of 77.56% at 45°C after 12 h | Penicillium aurantiogriseum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Penicillium aurantiogriseum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | the purified enzyme maintains over 60% of activity from pH 6.0 to 11.0 | Penicillium aurantiogriseum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | the purified enzyme does not lose any activity after 24 h at pH 6.0-11.0 | Penicillium aurantiogriseum |
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Release 2023.1 (January 2023)
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