Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.18 extracted from

  • Yang, Y.; Iwamoto, A.; Kumrungsee, T.; Okazaki, Y.; Kuroda, M.; Yamaguchi, S.; Kato, N.
    Consumption of an acid protease derived from Aspergillus oryzae causes bifidogenic effect in rats (2017), Nutr. Res., 44, 60-66 .
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q06902
-
-
Aspergillus oryzae ATCC 42149 Q06902
-
-
Aspergillus oryzae RIB 40 Q06902
-
-

Purification (Commentary)

Purification (Comment) Organism
the enzyme is purified by 46fold from an Amano protease preparation from Aspergillus oryzae Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation the enzyme is isolated from an Amano protease preparation from Aspergillus oryzae, enzyme AcP is purified from Biozyme A Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
242
-
purified enzyme, pH 3.0, temperature not specified in the publication Aspergillus oryzae

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
Acid protease
-
Aspergillus oryzae
ACP
-
Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
assay at Aspergillus oryzae

General Information

General Information Comment Organism
additional information analysis of N-terminal amino acid sequence indicated that the purified enzyme is an acid protease Aspergillus oryzae
physiological function the acid protease derived from Aspergillus oryzae causes bifidogenic effect in Sprague-Dawley rats. Bifidobacterium numbers are unaffected by supplementation with purified acid protease (AcP, 0.096 g/kg) at the level equivalent to the AcP amount found in the 1-g/kg Amano protease diet. Bifidobacterium numbers in the cecum and feces, and lactate levels in the cecum are significantly elevated when rats are fed a diet containing 0.384 g/kg AcP (4fold higher amount of AcP than that used in the 1-g/kg Amano protease diet) Aspergillus oryzae