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Literature summary for 3.4.23.18 extracted from

  • Deng, J.; Huang, W.; Li, Z.; Lu, D.; Zhang, Y.; Luo, X.
    Biocontrol activity of recombinant aspartic protease from Trichoderma harzianum against pathogenic fungi (2018), Enzyme Microb. Technol., 112, 35-42 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Trichoderma harzianum

Inhibitors

Inhibitors Comment Organism Structure
additional information EDTA has a negligible effect on the enzyme activity Trichoderma harzianum
pepstatin complete inhibition at 10 mM Trichoderma harzianum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation at 10 mM Trichoderma harzianum
Cu2+ 151.2% activity at 10 mM Trichoderma harzianum
Mg2+ slight activation at 10 mM Trichoderma harzianum
Mn2+ 140.1% activity at 10 mM Trichoderma harzianum
additional information Fe2+ and Zn2+ have negligible effect on the enzyme activity Trichoderma harzianum
Ni2+ slight activation at 10 mM Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum Q334I5
-
-
Trichoderma harzianum GIM 3.442 Q334I5
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex G-75 gel filtration Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Trichoderma harzianum ?
-
?
casein + H2O
-
Trichoderma harzianum GIM 3.442 ?
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Trichoderma harzianum

Synonyms

Synonyms Comment Organism
aspartic protease P6281
-
Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Trichoderma harzianum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 more than 50% activity between 25 and 45°C Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme maintains 39.8% of its maximum activity after 5 min incubation at 50°C. The protease remains stable after 1 h of incubation at 30 and 35°C, retaining more than 70% activity Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
Trichoderma harzianum

pH Range

pH Minimum pH Maximum Comment Organism
2 6 about 5% activity at pH 2.0, 100% activity at pH 2.5, about 95% activity at pH 3.0, about 60% activity at pH 3.5, about 50% activity at pH 4.0, about 15% activity at pH 5.0, about 3% activity at pH 6.0 Trichoderma harzianum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 6 the enzyme is stable in the pH range of 2.5-6.0, maintaining more than 80% of its activity after 1 h of incubation. Enzymatic activity is lost after incubation at pH 10.0 Trichoderma harzianum

General Information

General Information Comment Organism
physiological function the enzyme significantly inhibits spore germination and growth of plant and animal pathogenic fungi such as Botrytis cinerea, Mucor circinelloides, Aspergillus fumigatus, Aspergillus flavus, Rhizoctonia solani, and Candida albicans. The enzyme efficiently damages the cell wall of Botrytis cinerea. In addition, the protease significantly inhibits the development of grey mold that causes rotting of apple, orange, and cucumber Trichoderma harzianum