Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Trichoderma harzianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA has a negligible effect on the enzyme activity | Trichoderma harzianum | |
pepstatin | complete inhibition at 10 mM | Trichoderma harzianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation at 10 mM | Trichoderma harzianum | |
Cu2+ | 151.2% activity at 10 mM | Trichoderma harzianum | |
Mg2+ | slight activation at 10 mM | Trichoderma harzianum | |
Mn2+ | 140.1% activity at 10 mM | Trichoderma harzianum | |
additional information | Fe2+ and Zn2+ have negligible effect on the enzyme activity | Trichoderma harzianum | |
Ni2+ | slight activation at 10 mM | Trichoderma harzianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | Q334I5 | - |
- |
Trichoderma harzianum GIM 3.442 | Q334I5 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex G-75 gel filtration | Trichoderma harzianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Trichoderma harzianum | ? | - |
? | |
casein + H2O | - |
Trichoderma harzianum GIM 3.442 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Trichoderma harzianum |
Synonyms | Comment | Organism |
---|---|---|
aspartic protease P6281 | - |
Trichoderma harzianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Trichoderma harzianum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | more than 50% activity between 25 and 45°C | Trichoderma harzianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme maintains 39.8% of its maximum activity after 5 min incubation at 50°C. The protease remains stable after 1 h of incubation at 30 and 35°C, retaining more than 70% activity | Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Trichoderma harzianum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | about 5% activity at pH 2.0, 100% activity at pH 2.5, about 95% activity at pH 3.0, about 60% activity at pH 3.5, about 50% activity at pH 4.0, about 15% activity at pH 5.0, about 3% activity at pH 6.0 | Trichoderma harzianum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6 | the enzyme is stable in the pH range of 2.5-6.0, maintaining more than 80% of its activity after 1 h of incubation. Enzymatic activity is lost after incubation at pH 10.0 | Trichoderma harzianum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme significantly inhibits spore germination and growth of plant and animal pathogenic fungi such as Botrytis cinerea, Mucor circinelloides, Aspergillus fumigatus, Aspergillus flavus, Rhizoctonia solani, and Candida albicans. The enzyme efficiently damages the cell wall of Botrytis cinerea. In addition, the protease significantly inhibits the development of grey mold that causes rotting of apple, orange, and cucumber | Trichoderma harzianum |