Crystallization (Comment) | Organism |
---|---|
Aspergillus oryzae | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | diisopropyl phosphofluoridate; EDTA; HgCl2, NaF, FeCl3, FeCl2, CoCl2, MgCl2, CaCl2, ZnSO4; monoiodoacetate, 1,10-phenanthroline, 6-aminohexanoate; PCMB | Aspergillus oryzae | |
N-bromosuccinimide | not | Aspergillus oryzae | |
SDS | - |
Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by HgCl2, NaF, FeCl3, FeCl2, CoCl2, MgCl2, CaCl2, ZnSO4 | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Aspergillus oryzae |
32000 | - |
Aspergillus oryzae, enzyme form A2, sedimentation analysis | Aspergillus oryzae |
63000 | - |
Aspergillus oryzae, enzyme form A1, sedimetation analysis | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content: less than 0.3% (enzyme form A2), about 50% (enzyme form A1) | Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
several isoforms (extracellular A1 and A2) | Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | Taka-diastase, crude Aspergillus oryzae powder preparation | Aspergillus oryzae | - |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM sodium acetate, pH 5, stable | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Aspergillus oryzae | ? | - |
? | |
Cytochrome c + H2O | from horse heart | Aspergillus oryzae | ? | - |
? | |
Hemoglobin + H2O | urea-denatured hemoglobin | Aspergillus oryzae | ? | - |
? | |
Trypsinogen + H2O | bovine (pancreatic) | Aspergillus oryzae | ? | - |
? | |
Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, in 50 mM sodium acetate buffer, pH 5 | Aspergillus oryzae |
55 | - |
isozyme A1 loses 10% of its original activity in contrast to almost complete loss of activity of isozyme A2 | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 3.15 and 3.5 (isozyme A1) | Aspergillus oryzae |
additional information | - |
pI: 3.9 (isozyme A2) | Aspergillus oryzae |
3 | - |
cytochrome c | Aspergillus oryzae |
4.2 | - |
urea-denatured hemoglobin | Aspergillus oryzae |