Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
Q7 | mutant enzyme has markedly enhanced stability over the wild-type enzyme | Human immunodeficiency virus 1 |
Q7K/L331I/L631I | mutant enzyme is highly resistant to autolysis, while retaining the physical properties, specificity, and susceptibility to inhibition of the wild-type enzyme | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
U-71017 | i.e. Poa-His-Cha-PSI[CH(OH)CH2]Val-Ile-Amp | Human immunodeficiency virus 1 | |
U-71038 | i.e. Boc-Pro-Phe-Nalpha-MeHis-Leu-PSI[CH(OH)CH2]Val-Ile-Amp | Human immunodeficiency virus 1 | |
U-75875 | i.e. Noa-His-Cha-PSI[CH(OH)CH2]Val-Ile-Amp | Human immunodeficiency virus 1 | |
U-79213 | i.e. Poa-His-Cha-PSI[CH(OH)CH2]Val-Ile-Amp | Human immunodeficiency virus 1 | |
U-81749E | i.e. Tba-Cha-PSI[CH(OH)CH2]Val-Ile-Amp | Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
REYDQILIKVS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.135 | - |
REYDQILIKVS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.143 | - |
REYDQIIIEVS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.152 | - |
REYDQIIIEVS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.202 | - |
REYDQILIEVS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.215 | - |
REYDQILIEVS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.71 | - |
DTVIEEMS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.81 | - |
DTVIEEMS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.87 | - |
DTVVEEMS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.95 | - |
DTVVEEMS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
1.02 | - |
DTVLEEMS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
1.11 | - |
DTVLEEMS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
2 | - |
VSQNYPIV | pH 5.5, 30°C, wild-type enzyme and mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
2.2 | - |
PQITLWQRP | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inclusion body | inclusion bodies from Escherichia coli harboring recombinant HIV-1 | Human immunodeficiency virus 1 | 16234 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DTVIEEMS + H2O | - |
Human immunodeficiency virus 1 | DTVI + EEMS | - |
? | |
DTVLEEMS + H2O | - |
Human immunodeficiency virus 1 | DTVL + EEMS | - |
? | |
DTVVEEMS + H2O | - |
Human immunodeficiency virus 1 | DTVV + EEMS | - |
? | |
DTVVEKMS + H2O | - |
Human immunodeficiency virus 1 | DTVV + EKMS | - |
? | |
PQITLWKRP + H2O | - |
Human immunodeficiency virus 1 | PQITL + WKRP | - |
? | |
PQITLWQRP + H2O | - |
Human immunodeficiency virus 1 | PQITL + WQRP | - |
? | |
REYDQIIIEVS + H2O | - |
Human immunodeficiency virus 1 | REYDQII + IEVS | - |
? | |
REYDQILIEVS + H2O | - |
Human immunodeficiency virus 1 | REYDQIL + IEVS | - |
? | |
REYDQILIKVS + H2O | - |
Human immunodeficiency virus 1 | REYDQIL + IKVS | - |
? | |
VSQNYPIV + H2O | - |
Human immunodeficiency virus 1 | VSQNY + PIV | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the HIV-1 protease dimer is in equilibrium with monomeric, disordered species which serves as the substrate for autolysis | Human immunodeficiency virus 1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000833 | - |
REYDQILIKVS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.001 | - |
REYDQILIKVS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.025 | - |
REYDQIIIEVS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.0267 | - |
REYDQIIIEVS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.04 | - |
DTVIEEMS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.0483 | - |
DTVIEEMS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.0967 | - |
PQITLWQRP | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.103 | - |
PQITLWQRP | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.125 | - |
DTVVEEMS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
0.13 | - |
DTVVEEMS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
2.85 | - |
REYDQILIEVS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
3.07 | - |
REYDQILIEVS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
4.02 | - |
VSQNYPIV | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
4.32 | - |
VSQNYPIV | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 | |
4.35 | - |
DTVLEEMS | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
4.58 | - |
DTVLEEMS | pH 5.5, 30°C, mutant enzyme Q7K/L33I/L63I | Human immunodeficiency virus 1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000007 | - |
U-71017 | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.000009 | - |
U-79213 | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.000011 | - |
U-71038 | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 | |
0.00006 | - |
U-81749E | pH 5.5, 30°C, wild-type enzyme | Human immunodeficiency virus 1 |