Literature summary for 3.4.23.15 extracted from

Yamashita, S.; Shibata, N.; Boku-Ikeda, A.; Abe, E.; Inayama, A.; Yamaguchi, T.; Higuma, A.; Inagaki, K.; Tsuyuzaki, T.; Iwamoto, S.; Ohno, S.; Yokogawa, T.; Nishikawa, K.; Biswas, K.B.; Nabi, A.H.; Nakagawa, T.; Suzuki, F.; Ebihara, A.
Escherichia coli-based production of recombinant ovine angiotensinogen and its characterization as a renin substrate (2016), BMC Biotechnol., 16, 33 .

Search for more literature for 3.4.23.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in CHO cells	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000071	-	recombinant ovine angiotensinogen from Escherichia coli	at pH 7.2 and 37°C	Homo sapiens
0.000072	-	recombinant ovine angiotensinogen from CHO cells	at pH 7.2 and 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Resource S column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
recombinant ovine angiotensinogen from CHO cells + H2O	-	Homo sapiens	angiotensin I + angiotensinogen propeptide	-	?
recombinant ovine angiotensinogen from Escherichia coli + H2O	-	Homo sapiens	angiotensin I + angiotensinogen propeptide	-	?

Synonyms

Synonyms	Comment	Organism
prorenin	proenzyme	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.46	-	recombinant ovine angiotensinogen from CHO cells	at pH 7.2 and 37°C	Homo sapiens
0.59	-	recombinant ovine angiotensinogen from Escherichia coli	at pH 7.2 and 37°C	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6400	-	recombinant ovine angiotensinogen from CHO cells	at pH 7.2 and 37°C	Homo sapiens
8300	-	recombinant ovine angiotensinogen from Escherichia coli	at pH 7.2 and 37°C	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)