Any feedback?
Literature summary for 3.4.23.1 extracted from
- Purification and characterization of pepsinogens and pepsins from the stomach of rice field eel (Monopterus albus Zuiew) (2011), Fish Physiol. Biochem., 37, 543-552.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | pepsinogen is converted into pepsin quickly at pH 2.0 | Monopterus albus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by E-64 and EDTA | Monopterus albus | |
| pepstatin A | completely inhibited at a pepstatin/pepsin molar ratio of 1:2 | Monopterus albus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Monopterus albus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, active enzyme, SDS-PAGE | Monopterus albus |
| 36000 | - |
x * 36000, pepsinogen, SDS-PAGE | Monopterus albus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monopterus albus | - |
rice field eel | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sephacel gel filtration, and Sephacryl S-200 gel filtration | Monopterus albus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stomach | - |
Monopterus albus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activity of the crude extract and 8.4fold purified enzyme are 1.8 and 15.1 units/mg. One unit of pepsin activity is defined as an increase of absorbance of 1.0 at 280 nm during an incubation time of 30 min at pH 3.0 and 37°C | Monopterus albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hemoglobin + H2O | - |
Monopterus albus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, active enzyme, SDS-PAGE | Monopterus albus |
| ? | x * 36000, pepsinogen, SDS-PAGE | Monopterus albus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pepsin 1 | isoform | Monopterus albus |
| PG1 | pepsinogen isoform | Monopterus albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the activity of pepsin isoform 1 is largely lost at higher than 50°C | Monopterus albus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23.2 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Monopterus albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Monopterus albus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Monopterus albus | pepsinogen 1, isoelectric focusing | - |
5.1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 190 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Monopterus albus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
