Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.1 extracted from

  • Simoes, I.; Faro, R.; Bur, D.; Kay, J.; Faro, C.
    Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis (2011), FEBS J., 278, 3177-3186.
    View publication on PubMed
Search for more literature for 3.4.23.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Shewanella amazonensis

Protein Variants

Protein Variants Comment Organism
D37A the mutant is completely inactive towards the fluorogenic substrate Mca-KKPAEFFALK-Dnp at pH 4.0 Shewanella amazonensis

Inhibitors

Inhibitors Comment Organism Structure
amastatin 97.2 residual activity at 0.01 mM Shewanella amazonensis
bestatin 92.7 residual activity at 0.01 mM Shewanella amazonensis
dithiothreitol 91.2 residual activity at 2 mM Shewanella amazonensis
E-64 89.5 residual activity at 0.01 mM Shewanella amazonensis
EDTA 82.8 residual activity at 5 mM Shewanella amazonensis
iodoacetamide 82.1 residual activity at 0.05 mM Shewanella amazonensis
leupeptin 91.9 residual activity at 0.01 mM Shewanella amazonensis
pefabloc 87.8 residual activity at 1 mM Shewanella amazonensis
pepstatin A complete inhibition at 0.001 mM Shewanella amazonensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
 Mca-KKPAEFFALK-Dnp at pH 4.0 and 37°C Shewanella amazonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Shewanella amazonensis

Organism

Organism UniProt Comment Textmining
Shewanella amazonensis A1S3N8
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Shewanella amazonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mca-KKPAEFFALK-Dnp + H2O this peptide is preferentially cleaved at Phe-Phe, with a second minor cleavage occurring at Phe-Ala Shewanella amazonensis Mca-KKPAEF + FFALK-Dnp
-
 ?
Mca-KLHPEVLFVLEK-Dnp + H2O the preferred cleavage site is between Leu-Phe, a minor cleavage site is between Phe-Ala Shewanella amazonensis Mca-KLHPEVL + FVLEK-Dnp
-
 ?
additional information Edans-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys-Dabcyl-Arg, Arg-Glu-Edans-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys-Dabcyl-Arg, and Mca-Lys-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-Dnp are not significantly processed by shewasin A Shewanella amazonensis ?
-
 ?
oxidized insulin B chain + H2O two major cleavage sites are at Leu15-Tyr16 and Tyr16-Leu17 Shewanella amazonensis FVNQHLCGSHLVEAL + L-Tyr + LVCGERGFFYTPKA
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Shewanella amazonensis

Synonyms

Synonyms Comment Organism
shewasin A
-
 Shewanella amazonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42 50 maximum activity is observed at temperatures between 42 and 50°C, decreasing so drastically above 50°C that complete loss of activity is detected at 60°C Shewanella amazonensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50
-
 Shewanella amazonensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 Mca-KKPAEFFALK-Dnp at pH 4.0 and 37°C Shewanella amazonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8 4.5
-
 Shewanella amazonensis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5 50% activity is retained at pH 5.0 At pH 6.0, the enzyme shows no activity Shewanella amazonensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.6
-
 Mca-KKPAEFFALK-Dnp at pH 4.0 and 37°C Shewanella amazonensis