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Literature summary for 3.4.23.1 extracted from

  • Okoniewska, M.; Tanaka, T.; Yada, R.Y.
    The pepsin residue glycine-76 contributes to active-site loop flexibility and participates in catalysis (2000), Biochem. J., 349, 169-177.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli CJ236 Sus scrofa

Protein Variants

Protein Variants Comment Organism
G76A mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity Sus scrofa
G76S mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity Sus scrofa
G76V mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, wild-type enzyme Sus scrofa
0.04
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76A Sus scrofa
0.041
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76S Sus scrofa
0.043
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, wild-type enzyme Sus scrofa
0.044
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76V Sus scrofa
0.071
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76V Sus scrofa
0.085
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76S Sus scrofa
0.098
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76A Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu + H2O
-
Sus scrofa ?
-
?
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu + H2O
-
Sus scrofa ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76V Sus scrofa
2.5
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76S Sus scrofa
2.9
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76S Sus scrofa
11.1
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76V Sus scrofa
23.8
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, wild-type enzyme Sus scrofa
43.4
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, mutant enzyme G76A Sus scrofa
47
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, mutant enzyme G76A Sus scrofa
88.7
-
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu pH 3.95, 37°C, wild-type enzyme Sus scrofa
180.5
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, wild-type enzyme Sus scrofa
181
-
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu pH 2.1, 37°C, wild-type enzyme Sus scrofa