Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli CJ236 | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
G76A | mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity | Sus scrofa |
G76S | mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity | Sus scrofa |
G76V | mutant enzyme with lower catalytic efficiency and is activated more slowly than the wild-type enzyme. The slower activation process is associated directly with altered proteolytic activity | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, wild-type enzyme | Sus scrofa | |
0.04 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, mutant enzyme G76A | Sus scrofa | |
0.041 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, mutant enzyme G76S | Sus scrofa | |
0.043 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, wild-type enzyme | Sus scrofa | |
0.044 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, mutant enzyme G76V | Sus scrofa | |
0.071 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, mutant enzyme G76V | Sus scrofa | |
0.085 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, mutant enzyme G76S | Sus scrofa | |
0.098 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, mutant enzyme G76A | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu + H2O | - |
Sus scrofa | ? | - |
? | |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu + H2O | - |
Sus scrofa | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, mutant enzyme G76V | Sus scrofa | |
2.5 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, mutant enzyme G76S | Sus scrofa | |
2.9 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, mutant enzyme G76S | Sus scrofa | |
11.1 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, mutant enzyme G76V | Sus scrofa | |
23.8 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, wild-type enzyme | Sus scrofa | |
43.4 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, mutant enzyme G76A | Sus scrofa | |
47 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, mutant enzyme G76A | Sus scrofa | |
88.7 | - |
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu | pH 3.95, 37°C, wild-type enzyme | Sus scrofa | |
180.5 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, wild-type enzyme | Sus scrofa | |
181 | - |
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu | pH 2.1, 37°C, wild-type enzyme | Sus scrofa |