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Literature summary for 3.4.22.B79 extracted from

  • Rausalu, K.; Utt, A.; Quirin, T.; Varghese, F.S.; Zusinaite, E.; Das, P.K.; Ahola, T.; Merits, A.
    Chikungunya virus infectivity, RNA replication and non-structural polyprotein processing depend on the nsP2 proteases active site cysteine residue (2016), Sci. Rep., 6, 37124 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B79

Protein Variants

Protein Variants Comment Organism
C478A residue Cys478 in the active site of CHIKV nsP2 is indispensable for polyprotein P1234 processing. Mutation completely abolishes RNA replication trans-replication systems and abolishes the rescue of infectious virus from CHIKV RNA transcripts Chikungunya virus
S482A mutation has almost no negative effect on the protease activity Chikungunya virus
W479A mutation completely abolishes RNA replication trans-replication systems and abolishes the rescue of infectious virus from CHIKV RNA transcripts Chikungunya virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGGYIFSS-Edans 30°C, pH not specified in the publication Chikungunya virus

Organism

Organism UniProt Comment Textmining
Chikungunya virus Q1W368 nonstructural polyprotein
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Chikungunya virus ECSA Q1W368 nonstructural polyprotein
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGGYIFSS-Edans + H2O
-
 Chikungunya virus 4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGG + YIFSS-Edans
-
 ?
4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGGYIFSS-Edans + H2O
-
 Chikungunya virus ECSA 4-[[4-(dimethylamino)phenyl]-azo]benzoyl-DELRLDRAGG + YIFSS-Edans
-
 ?
4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGGYI-Edans + H2O peptide representing the 1/2 site of the polyprotein. Substrate is processed by nsP2, but the reaction velocity cannot be saturated Chikungunya virus 4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGG + YI-Edans
-
 ?
4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGGYI-Edans + H2O peptide representing the 1/2 site of the polyprotein. Substrate is processed by nsP2, but the reaction velocity cannot be saturated Chikungunya virus ECSA 4-[[4-(dimethylamino)phenyl]-azo]benzoyl-LDRAGG + YI-Edans
-
 ?
additional information protease readily cleaves GFP-10:5-Trx substrates containing short P10-P5' sequences originating from the 1/2 and 3/4 cleavage sites of the polyprotein, but not from the 2/3 site. Enzyme prefers the 3/4 substrate for cleavage in trans Chikungunya virus ?
-
 ?
additional information protease readily cleaves GFP-10:5-Trx substrates containing short P10-P5' sequences originating from the 1/2 and 3/4 cleavage sites of the polyprotein, but not from the 2/3 site. Enzyme prefers the 3/4 substrate for cleavage in trans Chikungunya virus ECSA ?
-
 ?