Literature summary for 3.4.22.B79 extracted from

Narwal, M.; Singh, H.; Pratap, S.; Malik, A.; Kuhn, R.J.; Kumar, P.; Tomar, S.
Crystal structure of chikungunya virus nsP2 cysteine protease reveals a putative flexible loop blocking its active site (2018), Int. J. Biol. Macromol., 116, 451-462 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Chikungunya virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.59 A resolution. Protein consists of an N-terminal protease subdomain and a C-terminal methyltransferase subdomain. The substrate binding cleft is present at the interface of two subdomains. Access to the active site and substrate binding cleft is blocked by a flexible interdomain loop. This loop contains His548, the catalytic residue, and Trp549 and Asn547, the residues predicted to bind substrate	Chikungunya virus

Crystallization (Comment)

Organism

to 2.59 A resolution. Protein consists of an N-terminal protease subdomain and a C-terminal methyltransferase subdomain. The substrate binding cleft is present at the interface of two subdomains. Access to the active site and substrate binding cleft is blocked by a flexible interdomain loop. This loop contains His548, the catalytic residue, and Trp549 and Asn547, the residues predicted to bind substrate

Chikungunya virus

Protein Variants

Protein Variants	Comment	Organism
C478A	mutation in active site, loss of activity	Chikungunya virus
N547A	mutation leads to three-fold increase in Km value	Chikungunya virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans	wild-type, pH not specified in the publication, temperature not specified in the publication	Chikungunya virus
0.029	-	4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans	mutant N547A, pH not specified in the publication, temperature not specified in the publication	Chikungunya virus

Organism

Organism	UniProt	Comment	Textmining
Chikungunya virus	Q8JUX6	-	-
Chikungunya virus S27-African prototype	Q8JUX6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans + H2O	-	Chikungunya virus	4-(4-dimethylaminophenyl-azo)benzoyl-DRAGG + YIFSE-Edans	-	?
4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans + H2O	-	Chikungunya virus S27-African prototype	4-(4-dimethylaminophenyl-azo)benzoyl-DRAGG + YIFSE-Edans	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Chikungunya virus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.14	-	4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans	mutant N547A, pH not specified in the publication, temperature not specified in the publication	Chikungunya virus
0.41	-	4-(4-dimethylaminophenyl-azo)benzoyl-DRAGGYIFSE-Edans	wild-type, pH not specified in the publication, temperature not specified in the publication	Chikungunya virus