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Literature summary for 3.4.22.B79 extracted from

  • Saisawang, C.; Kuadkitkan, A.; Smith, D.R.; Ubol, S.; Ketterman, A.J.
    Glutathionylation of chikungunya nsP2 protein affects protease activity (2017), Biochim. Biophys. Acta, 1861, 106-111 .
    View publication on PubMed
Search for more literature for 3.4.22.B79

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00016
-
 2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) presence of glutathione disulfide, pH 7.5, 37°C Chikungunya virus
0.00023
-
 2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) presence of glutathione disulfide, pH 7.5, 37°C Chikungunya virus
0.00043
-
 2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) pH 7.5, 37°C Chikungunya virus
0.00043
-
 2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) presence of glutathione disulfide, pH 7.5, 37°C Chikungunya virus
0.00057
-
 2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) pH 7.5, 37°C Chikungunya virus
0.00142
-
 2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) pH 7.5, 37°C Chikungunya virus

Organism

Organism UniProt Comment Textmining
Chikungunya virus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification in virus-infected cells, nsP2 can be glutathionylated at residues C175, C214, C313, C462, C698, C755. The glutathionylation of nsP2 impacts on the protease activity Chikungunya virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) + H2O corresponds to cleavage site P1/2 of the polyprotein Chikungunya virus 2-(N-methylamino)benzoyl-AGA + GIIETk(Dnp)
-
 ?
2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) + H2O corresponds to cleavage site P2/3 of the polyprotein Chikungunya virus 2-(N-methylamino)benzoyl-AGC + GIIETk(Dnp)
-
 ?
2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) + H2O corresponds to cleavage site P3/4 of the polyprotein Chikungunya virus 2-(N-methylamino)benzoyl-AGG + GIIETk(Dnp)
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00068
-
 2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) presence of glutathione disulfide, pH 7.5, 37°C Chikungunya virus
0.00084
-
 2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) presence of glutathione disulfide, pH 7.5, 37°C Chikungunya virus
0.00142
-
 2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) presence of glutathione disulfide, pH 7.5, 37°C Chikungunya virus
0.00182
-
 2-(N-methylamino)benzoyl-AGGGIIETk(Dnp) pH 7.5, 37°C Chikungunya virus
0.00281
-
 2-(N-methylamino)benzoyl-AGAGIIETk(Dnp) pH 7.5, 37°C Chikungunya virus
0.00445
-
 2-(N-methylamino)benzoyl-AGCGIIETk(Dnp) pH 7.5, 37°C Chikungunya virus