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Literature summary for 3.4.22.B79 extracted from

  • Hu, X.; Compton, J.R.; Leary, D.H.; Olson, M.A.; Lee, M.S.; Cheung, J.; Ye, W.; Ferrer, M.; Southall, N.; Jadhav, A.; Morazzani, E.M.; Glass, P.J.; Marugan, J.; Legler, P.M.
    Kinetic, mutational, and structural studies of the Venezuelan equine encephalitis virus nonstructural protein 2 cysteine protease (2016), Biochemistry, 55, 3007-3019 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Venezuelan equine encephalitis virus

Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and adduct with inhibitor E64d Venezuelan equine encephalitis virus

Protein Variants

Protein Variants Comment Organism
K480A mutation leads to a 9fold decrease in kcat and kcat/Km. K480 likely enhances the nucleophilicity of the Cys residue Venezuelan equine encephalitis virus
K706A SAM MTase domain mutation, increases Km 4.5fold to 500 microM Venezuelan equine encephalitis virus
N475A mutation leads to a 24fold reduction in kcat/Km, and the conformation of this residue does not change after inhibition. Residue N475 forms the base of the P1 binding site and likely orients the substrate for nucleophilic attack or plays a role in product release Venezuelan equine encephalitis virus
N545A mutation within the beta-hairpin, lightly but not significantly increases kcat and Km Venezuelan equine encephalitis virus
R662A mutation leads to 16fold decreases in kcat/Km Venezuelan equine encephalitis virus
R662K mutation leads to 16fold decreases in kcat/Km Venezuelan equine encephalitis virus

Inhibitors

Inhibitors Comment Organism Structure
E64d inhibitor binds beneath a beta-hairpin at the interface of the SAM MTase and protease domains Venezuelan equine encephalitis virus
additional information E64c, the carboxylic acid form of the E64d ester, does not inhibit the nsP2 protease Venezuelan equine encephalitis virus

Organism

Organism UniProt Comment Textmining
Venezuelan equine encephalitis virus P27282
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Venezuelan equine encephalitis virus Trinidad donkey P27282
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